Aims/hypothesis The beta cell transcriptional factor musculoaponeurotic fibrosarcoma oncogene family A (MafA) regulates genes important for beta cell function. Loss of nuclear MafA has been implicated in beta cell dysfunction in animal models of type 2 diabetes. We sought to establish if nuclear MafA is less abundant in beta cell nuclei in humans with type 2 diabetes. Methods Pancreas obtained at surgery from five nondiabetic individuals and six individuals with type 2 diabetes was immunostained for insulin, glucagon and MafA. Results Beta cell nuclear MafA was markedly decreased in type 2 diabetes (1.6±1.2% vs 46.3±8.3%, p<0.001). Conclusions/interpretation Beta cell nuclear MafA is markedly decreased in humans with type 2 diabetes, which may contribute to impaired beta cell dysfunction.
Bibliographical noteFunding Information:
Funding This study was funded by the National Institutes of Health (DK077967, DK059579, DK061539 to PCB and DK032585 to RPR) and the Larry L. Hillblom Foundation.
- Beta cell