BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells

Jin Song He, Ni Xie, Jian Bo Yang, Hong Guan, Wei Cai Chen, Chang Zou, Yi Wen Ouyang, You Sheng Mao, Xue Ying Luo, Yue Pan, Li Fu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Breast cancer-specific gene 1 (BCSG1), also referred to as γ-synuclein (SNCG), is highly expressed in human infiltrating breast carcinomas, but not in normal or benign breast tissue. The present study aimed to evaluate the effects of BCSG1 siRNA delivered by lentiviral vector on breast cancer cells and investigate the underlying mechanisms. BCSG1 RNAi lentiviral vector was constructed and transfected into MDA-MB-231 cells. BCSG1 mRNA levels were determined by quantitative polymerase chain reaction analysis. Cell proliferation, migration and apoptosis were evaluated by using the cell counting kit-8, Transwell assay and flow cytometry, respectively, followed by western blotting to determine the relative levels of AKT, extracellular signal-regulated kinase (ERK), p-AKT and p-ERK expression. BCSG1 mRNA levels were significantly reduced in MDA-MB-231 cells following transfection of BCSG1 siRNA delivered by lentiviral vector. Cell migration and proliferation were significantly decreased and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were significantly lower in the BCSG1 siRNAtreated groups compared with the control and negative control groups. Therefore, BCSG1 siRNA delivered by lentiviral vector was able to significantly reduce BCSG1 expression, suppress cell migration and proliferation, possibly through the reduction of the protein levels of p-AKT and p-ERK.

Original languageEnglish (US)
Pages (from-to)1659-1664
Number of pages6
JournalInternational journal of molecular medicine
Volume41
Issue number3
DOIs
StatePublished - Mar 2018

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Keywords

  • Breast cancer
  • Breast cancer-specific gene 1
  • Lentiviral vector
  • Small interfering RNA

Cite this

He, J. S., Xie, N., Yang, J. B., Guan, H., Chen, W. C., Zou, C., Ouyang, Y. W., Mao, Y. S., Luo, X. Y., Pan, Y., & Fu, L. (2018). BCSG1 siRNA delivered by lentiviral vector suppressed proliferation and migration of MDA-MB-231 cells. International journal of molecular medicine, 41(3), 1659-1664. https://doi.org/10.3892/ijmm.2017.3355