Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

Salwa Al-Mouqatea; Mohammad Alkhamis; Batool Akbar; Abdulmohsen Ali; Hameed Al-Aqeel; Ahmed Bin-Heji; Mohammed Razzaque; Julio Alvarez; Andres Perez

doi:10.1177/1040638718759574

Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait

Salwa Al-Mouqatea, Mohammad Alkhamis, Batool Akbar, Abdulmohsen Ali, Hameed Al-Aqeel, Ahmed Bin-Heji, Mohammed Razzaque, Julio Alvarez, Andres Perez

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.

Original language	English (US)
Pages (from-to)	468-470
Number of pages	3
Journal	Journal of Veterinary Diagnostic Investigation
Volume	30
Issue number	3
DOIs	https://doi.org/10.1177/1040638718759574
State	Published - May 1 2018

Bibliographical note

Funding Information:
The support provided by the management and other departments of the Kuwait Institute for Scientific Research is gratefully acknowledged. We thank the Public Authority for Agriculture Affairs and Fish Resources (PAAFR) and the Kuwait Union of Dairy Producers for technical cooperation provided during the study, and Dr. Y Al-Azemi for technical assistance. We are grateful to OMICS RU project SRUL02/13 in the RCF Research Core Facility at Kuwait University for the use of unit facilities.

Funding Information:
Research was funded by the Kuwait Foundation for the Advancement of Sciences (KFAS).

Keywords

Bovine tuberculosis
ELISA
Kuwait
gamma interferon
tuberculin

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Al-Mouqatea, S., Alkhamis, M., Akbar, B., Ali, A., Al-Aqeel, H., Bin-Heji, A., Razzaque, M., Alvarez, J., & Perez, A. (2018). Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait. Journal of Veterinary Diagnostic Investigation, 30(3), 468-470. https://doi.org/10.1177/1040638718759574

Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait. / Al-Mouqatea, Salwa; Alkhamis, Mohammad; Akbar, Batool; Ali, Abdulmohsen; Al-Aqeel, Hameed; Bin-Heji, Ahmed; Razzaque, Mohammed; Alvarez, Julio ; Perez, Andres.

In: Journal of Veterinary Diagnostic Investigation, Vol. 30, No. 3, 01.05.2018, p. 468-470.

Research output: Contribution to journal › Article › peer-review

Al-Mouqatea, S, Alkhamis, M, Akbar, B, Ali, A, Al-Aqeel, H, Bin-Heji, A, Razzaque, M, Alvarez, J & Perez, A 2018, 'Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait', Journal of Veterinary Diagnostic Investigation, vol. 30, no. 3, pp. 468-470. https://doi.org/10.1177/1040638718759574

Al-Mouqatea, Salwa ; Alkhamis, Mohammad ; Akbar, Batool ; Ali, Abdulmohsen ; Al-Aqeel, Hameed ; Bin-Heji, Ahmed ; Razzaque, Mohammed ; Alvarez, Julio ; Perez, Andres. / Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test–negative dairy cattle in Kuwait. In: Journal of Veterinary Diagnostic Investigation. 2018 ; Vol. 30, No. 3. pp. 468-470.

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abstract = "Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.",

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note = "Funding Information: The support provided by the management and other departments of the Kuwait Institute for Scientific Research is gratefully acknowledged. We thank the Public Authority for Agriculture Affairs and Fish Resources (PAAFR) and the Kuwait Union of Dairy Producers for technical cooperation provided during the study, and Dr. Y Al-Azemi for technical assistance. We are grateful to OMICS RU project SRUL02/13 in the RCF Research Core Facility at Kuwait University for the use of unit facilities. Funding Information: Research was funded by the Kuwait Foundation for the Advancement of Sciences (KFAS).",

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AU - Al-Aqeel, Hameed

AU - Bin-Heji, Ahmed

AU - Razzaque, Mohammed

AU - Alvarez, Julio

AU - Perez, Andres

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N2 - Bovine tuberculosis (TB) is endemic in Kuwait; cattle identified as TB-positive using the caudal fold test (CFT) are culled. We used a Bayesian approach to estimate the sensitivity (Se) and specificity (Sp) of the IFNγ assay and ELISA, which are not routinely used in Kuwait in CFT-negative dairy cattle. Blood samples from CFT-negative cattle (n = 384) collected from 38 dairy farms were tested by IFNγ assay and ELISA. The Se and Sp (95% CI) of the IFNγ were 85.0% (67.6–95.3%) and 90.4% (86.7–95.3%), respectively, whereas estimates for the ELISA were 61.1% (33.1–84.6%) and 85.4% (81.7–88.8%). TB prevalence (95 CI%) in CFT-negative cattle was estimated as 2.6% (0.5–9.5%). The IFNγ assay may play a role as an ancillary test for the identification of Mycobacterium bovis–infected cattle that are undetected by CFT.

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