Bax mitochondrial relocation is linked to its phosphorylation and its interaction with Bcl-xL

David Garenne, Thibaud T. Renault, Stéphen Manon

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system to investigate the molecular mechanisms underlying their interaction with mitochondria. By combining the co-expression of Bax and Bcl-xL mutants with analyzes of their localization and interaction in mitochondria and post-mitochondrial supernatants, we showed that the ability of Bax and Bcl-xL to interact is dependent both on Bax phosphorylation-mimicked by a substitution S184D-and by Bax and Bcl-xL localization. This, and previous data, provide the molecular basis for a model of dynamic equilibrium for Bax localization and activation, regulated both by phosphorylation and Bcl-xL.

Original languageEnglish (US)
Pages (from-to)597-605
Number of pages9
JournalMicrobial Cell
Volume3
Issue number12
DOIs
StatePublished - Dec 2016
Externally publishedYes

Keywords

  • Apoptosis
  • Bax
  • Bcl-xL
  • Cytochrome c release
  • Mitochondria
  • Phosphorylation
  • Yeast (S.cerevisiae)

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