Abstract
The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system to investigate the molecular mechanisms underlying their interaction with mitochondria. By combining the co-expression of Bax and Bcl-xL mutants with analyzes of their localization and interaction in mitochondria and post-mitochondrial supernatants, we showed that the ability of Bax and Bcl-xL to interact is dependent both on Bax phosphorylation-mimicked by a substitution S184D-and by Bax and Bcl-xL localization. This, and previous data, provide the molecular basis for a model of dynamic equilibrium for Bax localization and activation, regulated both by phosphorylation and Bcl-xL.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 597-605 |
| Number of pages | 9 |
| Journal | Microbial Cell |
| Volume | 3 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2016 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the Centre National de la Recherche Scientifique (UMR5095), the Université of Bordeaux, and the Agence Nationale de la Recherche (Project 'Phosbax').
Publisher Copyright:
© 2016 Garenne et al.
Keywords
- Apoptosis
- Bax
- Bcl-xL
- Cytochrome c release
- Mitochondria
- Phosphorylation
- Yeast (S.cerevisiae)