Barium reverses the transretinal potassium gradient of the amphibian retina

Paul A. Coleman; Peter L. Carras; Robert F. Miller

doi:10.1016/0304-3940(87)90495-2

Barium reverses the transretinal potassium gradient of the amphibian retina

Paul A. Coleman, Peter L. Carras, Robert F. Miller

Neuroscience

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Barium chloride (Ba²⁺) was added to the bathing medium of the perfused retina-eyecup preparation of the tiger salamander. The electroretinogram (ERG) and intraretinal extracellular potassium activity ([K⁺]_o) were analyzed using a double-barrel electrode, one of which was ion-selective for K⁺. The action of Ba²⁺ on the ERG was to attenuate the c-wave/slow PIII complex. In addition, Ba²⁺ dramatically decreased the [K⁺]_o in the outer retina, enough to reverse the transretinal potassium gradient. These findings, together with the known properties of Müller cell K⁺ channels, form the basis of an explanation of why Ba²⁺ blocks slow PIII and not the b-wave of the ERG.

Original language	English (US)
Pages (from-to)	61-65
Number of pages	5
Journal	Neuroscience Letters
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(87)90495-2
State	Published - Sep 11 1987

Bibliographical note

Funding Information:
This work was supportedb y NEI Grant EY008844 and NSF57900t o R.F.M. and NEI trainingG rantEY07057 to P.A.C. and P.L.C.

Keywords

Barium
Electroretinogram
Potassium-selective electrode
Retina
Tiger salamander

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10.1016/0304-3940(87)90495-2

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title = "Barium reverses the transretinal potassium gradient of the amphibian retina",

abstract = "Barium chloride (Ba2+) was added to the bathing medium of the perfused retina-eyecup preparation of the tiger salamander. The electroretinogram (ERG) and intraretinal extracellular potassium activity ([K+]o) were analyzed using a double-barrel electrode, one of which was ion-selective for K+. The action of Ba2+ on the ERG was to attenuate the c-wave/slow PIII complex. In addition, Ba2+ dramatically decreased the [K+]o in the outer retina, enough to reverse the transretinal potassium gradient. These findings, together with the known properties of M{\"u}ller cell K+ channels, form the basis of an explanation of why Ba2+ blocks slow PIII and not the b-wave of the ERG.",

keywords = "Barium, Electroretinogram, Potassium-selective electrode, Retina, Tiger salamander",

author = "Coleman, {Paul A.} and Carras, {Peter L.} and Miller, {Robert F.}",

note = "Funding Information: This work was supportedb y NEI Grant EY008844 and NSF57900t o R.F.M. and NEI trainingG rantEY07057 to P.A.C. and P.L.C.",

year = "1987",

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T1 - Barium reverses the transretinal potassium gradient of the amphibian retina

AU - Coleman, Paul A.

AU - Carras, Peter L.

AU - Miller, Robert F.

N1 - Funding Information: This work was supportedb y NEI Grant EY008844 and NSF57900t o R.F.M. and NEI trainingG rantEY07057 to P.A.C. and P.L.C.

PY - 1987/9/11

Y1 - 1987/9/11

N2 - Barium chloride (Ba2+) was added to the bathing medium of the perfused retina-eyecup preparation of the tiger salamander. The electroretinogram (ERG) and intraretinal extracellular potassium activity ([K+]o) were analyzed using a double-barrel electrode, one of which was ion-selective for K+. The action of Ba2+ on the ERG was to attenuate the c-wave/slow PIII complex. In addition, Ba2+ dramatically decreased the [K+]o in the outer retina, enough to reverse the transretinal potassium gradient. These findings, together with the known properties of Müller cell K+ channels, form the basis of an explanation of why Ba2+ blocks slow PIII and not the b-wave of the ERG.

AB - Barium chloride (Ba2+) was added to the bathing medium of the perfused retina-eyecup preparation of the tiger salamander. The electroretinogram (ERG) and intraretinal extracellular potassium activity ([K+]o) were analyzed using a double-barrel electrode, one of which was ion-selective for K+. The action of Ba2+ on the ERG was to attenuate the c-wave/slow PIII complex. In addition, Ba2+ dramatically decreased the [K+]o in the outer retina, enough to reverse the transretinal potassium gradient. These findings, together with the known properties of Müller cell K+ channels, form the basis of an explanation of why Ba2+ blocks slow PIII and not the b-wave of the ERG.

KW - Barium

KW - Electroretinogram

KW - Potassium-selective electrode

KW - Retina

KW - Tiger salamander

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EP - 65

JO - Neuroscience Letters

JF - Neuroscience Letters

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ER -

Barium reverses the transretinal potassium gradient of the amphibian retina

Abstract

Bibliographical note

Keywords

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