Bamboo (Phyllostachys pubescens) as a Natural Support for Neutral Protease Immobilization

Lei Peng Cao, Jing Jing Wang, Ting Zhou, R. R Ruan, Yu Huan Liu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lignin polymers in bamboo (Phyllostachys pubescens) were decomposed into polyphenols at high temperatures and oxidized for the introduction of quinone groups from peroxidase extracted from bamboo shoots and catalysis of UV. According to the results of FT-IR spectra analysis, neutral proteases (NPs) can be immobilized on the oxidized lignin by covalent bonding formed by amine group and quinone group. The optimum condition for the immobilization of NPs on the bamboo bar was obtained at pH 7.0, 40 °C, and duration of 4 h; the amount of immobilized enzyme was up to 5 mg g−1 bamboo bar. The optimal pH for both free NP (FNP) and INP was approximately 7.0, and the maximum activity of INP was determined at 60 °C, whereas FNP presented maximum activity at 50 °C. The Km values of INP and FNP were determined as 0.773 and 0.843 mg ml−1, respectively; INP showed a lower Km value and Vmax, than FNP, which demonstrated that INP presented higher affinity to substrate. Compared to FNP, INP showed broader thermal and storage stability under the same trial condition. With respect to cost, INP presented considerable recycling efficiency for up to six consecutive cycles.

Original languageEnglish (US)
Pages (from-to)109-121
Number of pages13
JournalApplied Biochemistry and Biotechnology
Volume186
Issue number1
DOIs
StatePublished - Sep 1 2018

Fingerprint

Bamboo
Immobilization
Peptide Hydrolases
Lignin
Immobilized Enzymes
Recycling
Polyphenols
Catalysis
Peroxidase
Amines
Spectrum Analysis
Polymers
Hot Temperature
Spectrum analysis
Costs and Cost Analysis
Temperature
Enzymes
Substrates
Costs
benzoquinone

Keywords

  • Bamboo
  • Covalent binding
  • Immobilization
  • Lignin
  • Neutral protease (NP)

PubMed: MeSH publication types

  • Journal Article

Cite this

Bamboo (Phyllostachys pubescens) as a Natural Support for Neutral Protease Immobilization. / Cao, Lei Peng; Wang, Jing Jing; Zhou, Ting; Ruan, R. R; Liu, Yu Huan.

In: Applied Biochemistry and Biotechnology, Vol. 186, No. 1, 01.09.2018, p. 109-121.

Research output: Contribution to journalArticle

Cao, Lei Peng ; Wang, Jing Jing ; Zhou, Ting ; Ruan, R. R ; Liu, Yu Huan. / Bamboo (Phyllostachys pubescens) as a Natural Support for Neutral Protease Immobilization. In: Applied Biochemistry and Biotechnology. 2018 ; Vol. 186, No. 1. pp. 109-121.
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