Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation

Eunice Essel; Junhong Xie; Chaochao Deng; Zhengkai Peng; Jinbin Wang; Jicheng Shen; Jianhui Xie; Jeffrey A. Coulter; Lingling Li

doi:10.1016/j.still.2019.104302

Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation

Eunice Essel, Junhong Xie, Chaochao Deng, Zhengkai Peng, Jinbin Wang, Jicheng Shen, Jianhui Xie, Jeffrey A. Coulter, Lingling Li

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Soil microbial diversity is important in maintaining soil quality, sustainable agriculture, and ecosystem function. Soil management practices can influence the diversity and activity of soil microbes in agricultural fields. The objective of this study was to assess the effects of tillage and stubble management on the diversity of bacteria and fungi, chemical property and total carbon emission in the rhizosphere and bulk soils in wheat (Triticum aestivum L.) -pea (Pisum arvense L.) rotation at pre-harvest. Treatments included conventional tillage with stubble removed (T), no-tillage with stubble removed (NT), conventional tillage with stubble incorporated (TS), and no-tillage with stubble retained (NTS). Bacteria 16S rRNA (V3V4) and fungi ITS (ITS2) region genes were sequenced from bulk soil and rhizosphere soils. Abundance of the dominant bacterial (Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Planctomycetes) and fungal (Ascomycota and Basidiomycota) phyla identified did not differ significantly (P < 0.05) among treatments. Rhizobium and rare fungi Kurtzmanomyces occurred in the rhizosphere but were virtually absent in bulk soil. However, bacterial and fungal OTUs diversity indices were less in the rhizosphere compared to bulk soil. Soil from the NTS and NT had the greatest bacteria 16S rRNA and fungi ITS region number of OTUs; however, the microbial community did not differ among treatments. The abundance of class level bacteria and fungi were associated with soil pH, total organic carbon, total nitrogen, NH₄ ⁺ -N, and NO₃ ^¯ -N contents. The results indicate that bulk soil had higher microbial library, which will be beneficial for establishment of the next season's microbial community.

Original language	English (US)
Article number	104302
Journal	Soil and Tillage Research
Volume	194
DOIs	https://doi.org/10.1016/j.still.2019.104302
State	Published - Nov 2019

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (grants 31460337 , 31660373 , and 31761143004 ), the National Twelfth Five-Year Plan Circular Agricultural Science and Technology Project (grant 2012 BAD14B03 ), the Education Department of Gansu Province (grant 2017C-12 ), and the Gansu Provincial Key Laboratory of Aridland Crop Science Open Fund Project (grant GSCS-2013-13 ). We appreciate assistance in the field and laboratory by students of the Dingxi Long-term Rainfed Agricultural Experimental Station of Gansu Agricultural University Agricultural.

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

16S rRNA
ITS region
Microbial diversity
Stubble
Tillage

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.still.2019.104302

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title = "Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation",

abstract = "Soil microbial diversity is important in maintaining soil quality, sustainable agriculture, and ecosystem function. Soil management practices can influence the diversity and activity of soil microbes in agricultural fields. The objective of this study was to assess the effects of tillage and stubble management on the diversity of bacteria and fungi, chemical property and total carbon emission in the rhizosphere and bulk soils in wheat (Triticum aestivum L.) -pea (Pisum arvense L.) rotation at pre-harvest. Treatments included conventional tillage with stubble removed (T), no-tillage with stubble removed (NT), conventional tillage with stubble incorporated (TS), and no-tillage with stubble retained (NTS). Bacteria 16S rRNA (V3V4) and fungi ITS (ITS2) region genes were sequenced from bulk soil and rhizosphere soils. Abundance of the dominant bacterial (Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Planctomycetes) and fungal (Ascomycota and Basidiomycota) phyla identified did not differ significantly (P < 0.05) among treatments. Rhizobium and rare fungi Kurtzmanomyces occurred in the rhizosphere but were virtually absent in bulk soil. However, bacterial and fungal OTUs diversity indices were less in the rhizosphere compared to bulk soil. Soil from the NTS and NT had the greatest bacteria 16S rRNA and fungi ITS region number of OTUs; however, the microbial community did not differ among treatments. The abundance of class level bacteria and fungi were associated with soil pH, total organic carbon, total nitrogen, NH4 + -N, and NO3 ¯ -N contents. The results indicate that bulk soil had higher microbial library, which will be beneficial for establishment of the next season's microbial community.",

keywords = "16S rRNA, ITS region, Microbial diversity, Stubble, Tillage",

author = "Eunice Essel and Junhong Xie and Chaochao Deng and Zhengkai Peng and Jinbin Wang and Jicheng Shen and Jianhui Xie and Coulter, {Jeffrey A.} and Lingling Li",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (grants 31460337 , 31660373 , and 31761143004 ), the National Twelfth Five-Year Plan Circular Agricultural Science and Technology Project (grant 2012 BAD14B03 ), the Education Department of Gansu Province (grant 2017C-12 ), and the Gansu Provincial Key Laboratory of Aridland Crop Science Open Fund Project (grant GSCS-2013-13 ). We appreciate assistance in the field and laboratory by students of the Dingxi Long-term Rainfed Agricultural Experimental Station of Gansu Agricultural University Agricultural. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

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doi = "10.1016/j.still.2019.104302",

language = "English (US)",

volume = "194",

journal = "Soil and Tillage Research",

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AU - Essel, Eunice

AU - Xie, Junhong

AU - Deng, Chaochao

AU - Peng, Zhengkai

AU - Wang, Jinbin

AU - Shen, Jicheng

AU - Xie, Jianhui

AU - Coulter, Jeffrey A.

AU - Li, Lingling

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (grants 31460337 , 31660373 , and 31761143004 ), the National Twelfth Five-Year Plan Circular Agricultural Science and Technology Project (grant 2012 BAD14B03 ), the Education Department of Gansu Province (grant 2017C-12 ), and the Gansu Provincial Key Laboratory of Aridland Crop Science Open Fund Project (grant GSCS-2013-13 ). We appreciate assistance in the field and laboratory by students of the Dingxi Long-term Rainfed Agricultural Experimental Station of Gansu Agricultural University Agricultural. Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/11

Y1 - 2019/11

N2 - Soil microbial diversity is important in maintaining soil quality, sustainable agriculture, and ecosystem function. Soil management practices can influence the diversity and activity of soil microbes in agricultural fields. The objective of this study was to assess the effects of tillage and stubble management on the diversity of bacteria and fungi, chemical property and total carbon emission in the rhizosphere and bulk soils in wheat (Triticum aestivum L.) -pea (Pisum arvense L.) rotation at pre-harvest. Treatments included conventional tillage with stubble removed (T), no-tillage with stubble removed (NT), conventional tillage with stubble incorporated (TS), and no-tillage with stubble retained (NTS). Bacteria 16S rRNA (V3V4) and fungi ITS (ITS2) region genes were sequenced from bulk soil and rhizosphere soils. Abundance of the dominant bacterial (Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Planctomycetes) and fungal (Ascomycota and Basidiomycota) phyla identified did not differ significantly (P < 0.05) among treatments. Rhizobium and rare fungi Kurtzmanomyces occurred in the rhizosphere but were virtually absent in bulk soil. However, bacterial and fungal OTUs diversity indices were less in the rhizosphere compared to bulk soil. Soil from the NTS and NT had the greatest bacteria 16S rRNA and fungi ITS region number of OTUs; however, the microbial community did not differ among treatments. The abundance of class level bacteria and fungi were associated with soil pH, total organic carbon, total nitrogen, NH4 + -N, and NO3 ¯ -N contents. The results indicate that bulk soil had higher microbial library, which will be beneficial for establishment of the next season's microbial community.

AB - Soil microbial diversity is important in maintaining soil quality, sustainable agriculture, and ecosystem function. Soil management practices can influence the diversity and activity of soil microbes in agricultural fields. The objective of this study was to assess the effects of tillage and stubble management on the diversity of bacteria and fungi, chemical property and total carbon emission in the rhizosphere and bulk soils in wheat (Triticum aestivum L.) -pea (Pisum arvense L.) rotation at pre-harvest. Treatments included conventional tillage with stubble removed (T), no-tillage with stubble removed (NT), conventional tillage with stubble incorporated (TS), and no-tillage with stubble retained (NTS). Bacteria 16S rRNA (V3V4) and fungi ITS (ITS2) region genes were sequenced from bulk soil and rhizosphere soils. Abundance of the dominant bacterial (Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Planctomycetes) and fungal (Ascomycota and Basidiomycota) phyla identified did not differ significantly (P < 0.05) among treatments. Rhizobium and rare fungi Kurtzmanomyces occurred in the rhizosphere but were virtually absent in bulk soil. However, bacterial and fungal OTUs diversity indices were less in the rhizosphere compared to bulk soil. Soil from the NTS and NT had the greatest bacteria 16S rRNA and fungi ITS region number of OTUs; however, the microbial community did not differ among treatments. The abundance of class level bacteria and fungi were associated with soil pH, total organic carbon, total nitrogen, NH4 + -N, and NO3 ¯ -N contents. The results indicate that bulk soil had higher microbial library, which will be beneficial for establishment of the next season's microbial community.

KW - 16S rRNA

KW - ITS region

KW - Microbial diversity

KW - Stubble

KW - Tillage

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M3 - Article

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SN - 0167-1987

VL - 194

JO - Soil and Tillage Research

JF - Soil and Tillage Research

M1 - 104302

ER -

Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation

Abstract

Bibliographical note

Keywords

UN SDGs

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