Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)

Yanfei Wang; Jinling Wang; Xiaoran Fu; Jeffrey R. Nageotte; Jennifer Silverman; Eric C. Bretsnyder; Danqi Chen; Timothy J. Rydel; Gregory J. Bean; Ke Sherry Li; Edward Kraft; Anilkumar Gowda; Autumn Nance; Robert G. Moore; Michael J. Pleau; Jason S. Milligan; Heather M. Anderson; Peter Asiimwe; Adam Evans; William J. Moar; Samuel Martinelli; Graham P. Head; Jeffrey A. Haas; James A. Baum; Fei Yang; David L. Kerns; Agoston Jerga

doi:10.1128/AEM.00579-19

Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)

Yanfei Wang
, Jinling Wang
, Xiaoran Fu
, Jeffrey R. Nageotte
, Jennifer Silverman
, Eric C. Bretsnyder
, Danqi Chen
, Timothy J. Rydel
, Gregory J. Bean
, Ke Sherry Li
, Edward Kraft
, Anilkumar Gowda
, Autumn Nance
, Robert G. Moore
, Michael J. Pleau
, Jason S. Milligan
, Heather M. Anderson
, Peter Asiimwe
, Adam Evans
, William J. Moar

Samuel Martinelli, Graham P. Head, Jeffrey A. Haas, James A. Baum, Fei Yang, David L. Kerns, Agoston Jerga

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.

Original language	English (US)
Article number	e00579-19
Journal	Applied and environmental microbiology
Volume	85
Issue number	16
DOIs	https://doi.org/10.1128/AEM.00579-19
State	Published - Aug 1 2019
Externally published	Yes

Bibliographical note

Funding Information:
Funding for this research was provided by Bayer Crop Science. We thank our current and former colleagues Randall Kerstetter, Melissa Kemp, Sara Salvador, and Crystal Dart at the Bayer Plant Biotechnology site in Cambridge, MA, for technical support. We also give special thanks to Cara Vazquez for her critical review of the manuscript and to Nancy Adams at the Bayer Insectary in Union City, TN. We also thank Jiexin Peng and her protein expression and purification team and David Duda and his team for continued structural biology support.

Publisher Copyright:
© 2019 American Society for Microbiology.

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SDG 2 Zero Hunger

Keywords

Bacillus thuringiensis
Bt toxin
Cry1B.868
Cry1Da_7
Disabled insecticidal protein
Insect resistance
Mechanisms of action
Mode of action

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10.1128/AEM.00579-19

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Wang, Y., Wang, J., Fu, X., Nageotte, J. R., Silverman, J., Bretsnyder, E. C., Chen, D., Rydel, T. J., Bean, G. J., Li, K. S., Kraft, E., Gowda, A., Nance, A., Moore, R. G., Pleau, M. J., Milligan, J. S., Anderson, H. M., Asiimwe, P., Evans, A., ... Jerga, A. (2019). Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith). Applied and environmental microbiology, 85(16), Article e00579-19. https://doi.org/10.1128/AEM.00579-19

Wang, Y, Wang, J, Fu, X, Nageotte, JR, Silverman, J, Bretsnyder, EC, Chen, D, Rydel, TJ, Bean, GJ, Li, KS, Kraft, E, Gowda, A, Nance, A, Moore, RG, Pleau, MJ, Milligan, JS, Anderson, HM, Asiimwe, P, Evans, A, Moar, WJ, Martinelli, S, Head, GP, Haas, JA, Baum, JA, Yang, F, Kerns, DL & Jerga, A 2019, 'Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)', Applied and environmental microbiology, vol. 85, no. 16, e00579-19. https://doi.org/10.1128/AEM.00579-19

@article{e7db033c5f924d5c9979c4f173380692,

title = "Bacillus thuringiensis Cry1Da\_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)",

abstract = "Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da\_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da\_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.",

keywords = "Bacillus thuringiensis, Bt toxin, Cry1B.868, Cry1Da\_7, Disabled insecticidal protein, Insect resistance, Mechanisms of action, Mode of action",

author = "Yanfei Wang and Jinling Wang and Xiaoran Fu and Nageotte, \{Jeffrey R.\} and Jennifer Silverman and Bretsnyder, \{Eric C.\} and Danqi Chen and Rydel, \{Timothy J.\} and Bean, \{Gregory J.\} and Li, \{Ke Sherry\} and Edward Kraft and Anilkumar Gowda and Autumn Nance and Moore, \{Robert G.\} and Pleau, \{Michael J.\} and Milligan, \{Jason S.\} and Anderson, \{Heather M.\} and Peter Asiimwe and Adam Evans and Moar, \{William J.\} and Samuel Martinelli and Head, \{Graham P.\} and Haas, \{Jeffrey A.\} and Baum, \{James A.\} and Fei Yang and Kerns, \{David L.\} and Agoston Jerga",

note = "Funding Information: Funding for this research was provided by Bayer Crop Science. We thank our current and former colleagues Randall Kerstetter, Melissa Kemp, Sara Salvador, and Crystal Dart at the Bayer Plant Biotechnology site in Cambridge, MA, for technical support. We also give special thanks to Cara Vazquez for her critical review of the manuscript and to Nancy Adams at the Bayer Insectary in Union City, TN. We also thank Jiexin Peng and her protein expression and purification team and David Duda and his team for continued structural biology support. Publisher Copyright: {\textcopyright} 2019 American Society for Microbiology.",

year = "2019",

month = aug,

day = "1",

doi = "10.1128/AEM.00579-19",

language = "English (US)",

volume = "85",

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T1 - Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)

AU - Wang, Yanfei

AU - Wang, Jinling

AU - Fu, Xiaoran

AU - Nageotte, Jeffrey R.

AU - Silverman, Jennifer

AU - Bretsnyder, Eric C.

AU - Chen, Danqi

AU - Rydel, Timothy J.

AU - Bean, Gregory J.

AU - Li, Ke Sherry

AU - Kraft, Edward

AU - Gowda, Anilkumar

AU - Nance, Autumn

AU - Moore, Robert G.

AU - Pleau, Michael J.

AU - Milligan, Jason S.

AU - Anderson, Heather M.

AU - Asiimwe, Peter

AU - Evans, Adam

AU - Moar, William J.

AU - Martinelli, Samuel

AU - Head, Graham P.

AU - Haas, Jeffrey A.

AU - Baum, James A.

AU - Yang, Fei

AU - Kerns, David L.

AU - Jerga, Agoston

N1 - Funding Information: Funding for this research was provided by Bayer Crop Science. We thank our current and former colleagues Randall Kerstetter, Melissa Kemp, Sara Salvador, and Crystal Dart at the Bayer Plant Biotechnology site in Cambridge, MA, for technical support. We also give special thanks to Cara Vazquez for her critical review of the manuscript and to Nancy Adams at the Bayer Insectary in Union City, TN. We also thank Jiexin Peng and her protein expression and purification team and David Duda and his team for continued structural biology support. Publisher Copyright: © 2019 American Society for Microbiology.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.

AB - Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.

KW - Bacillus thuringiensis

KW - Bt toxin

KW - Cry1B.868

KW - Cry1Da_7

KW - Disabled insecticidal protein

KW - Insect resistance

KW - Mechanisms of action

KW - Mode of action

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UR - https://www.scopus.com/pages/publications/85071054773#tab=citedBy

U2 - 10.1128/AEM.00579-19

DO - 10.1128/AEM.00579-19

M3 - Article

C2 - 31175187

AN - SCOPUS:85071054773

SN - 0099-2240

VL - 85

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

IS - 16

M1 - e00579-19

ER -

Bacillus thuringiensis Cry1Da_7 and Cry1B.868 protein interactions with novel receptors allow control of resistant fall armyworms, Spodoptera frugiperda (J.E. Smith)

Abstract

Bibliographical note

UN SDGs

Keywords

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