Bacillus megaterium strains derived from water and soil exhibit differential responses to the herbicide mesotrione

Tatiane Dobrzanski, Fernanda Gravina, Bruna Steckling, Luiz R. Olchanheski, Ricardo F. Sprenger, Bruno C. Espírito Santo, Carolina W. Galvão, Péricles M. Reche, Rosilene A. Prestes, Sônia A.V. Pileggi, Francinete R. Campos, Ricardo A. Azevedo, Michael J. Sadowsky, Flávio L. Beltrame, Marcos Pileggi

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

Original languageEnglish (US)
Article numbere0196166
JournalPloS one
Issue number4
StatePublished - Apr 2018

Bibliographical note

Funding Information:
This study was funded by Coordination for the Improvement of Higher Level Personnel (CAPES) (; National Council of Technological and Scientific Development (CNPq) (, Universal (Grant number: 445083/2014-0) and Science without Borders (Grant number: 401354/ 2014-8); Foundation for Research Support of the State of São Paulo (FAPESP) (http://www.fapesp. br/); and Foundation for Research Support of the State of Paraná (Fundação Araucária) (http://www. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors thank Maria Janina Pinheiro Diniz for assisting the preparation and the execution of experiments.

Publisher Copyright:
© 2018 Dobrzanski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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