B-cell lymphoproliferative disorders in solid-organ transplant patients: Detection of Epstein-Barr virus by in situ hybridization

Laurence C. Berg, Cedith M. Copenhaver, Vicki A. Morrison, Scott A. Gruber, David L. Dunn, K. Gajl-Peczalska, John G. Strickler

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34 Scopus citations


B-cell lymphoproliferative disorders (BLPDs) occur in approximately 2% of transplant recipients and are frequently fatal. Indirect serologic evidence has implicated Epstein-Barr virus (EBV) as an etiologic factor in these lesions. Direct evidence of the presence of EBV in these lesions has been obtained in relatively few cases. We used in situ hybridization (ISH) with a probe for the BamHI-W region of the EBV genome to study 52 tissue specimens from 28 solidorgan transplant patients who had BLPD. Epstein-Barr virus-infected lymphoid cells were identified in 26 of these 28 patients. The two patients without ISH evidence of EBV infection showed no distinctive clinical, morphologic, or serologic features. Previous filter-hybridization studies of these two patients had demonstrated evidence of EBV infection. Seven additional transplant patients without evidence of BLPD were studied as controls and showed no evidence of EBV in their lymphoid cells by ISH. These data provide further support for the etiologic role of EBV in the pathogenesis of posttransplantation lymphoproliferative disorders.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalHuman pathology
Issue number2
StatePublished - Feb 1992

Bibliographical note

Funding Information:
Fn,nr thr Drpwtments of Pathology. Medicine, and Surgery. liniversit) of Minnesota, Minneapolis, MN: and the Department of P,~thology, Wilford Hal1 Medical Center. Lackland Air Force Base. TN. Accepted for publication April 24, 199 1. Supported in part by a grant from the Minnesota Medical Foundation (J. G. 5%).n -search grant AM 13083 from the National Institutes of Health, and a grant from Sandoz Pharmaceuticals (Lxporation (5. A. G.). &T uro,dx Epstein-Barr virus, in situ hybridization, posttrans-plantation lymphoproliferative disorder. ‘The views expressed in this article are those of the authors and do not reflect the official policy of the Department of Defense or other departments of the LJS government. .Addres!, correspondence and reprint requests to John (;. Strickler. MD. Section of Surgical Pathology. Mayo Clinic and Mayo Foundation. Rochester. MN 5590.5. Copyright 6 1W ? by W.B. Saunders Compmy 0046-81 77/O”,CIO?-00 11$5.00/0


  • Epstein-Barr virus
  • in situ hybridization
  • posttransplantation lymphoproliferative disorder


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