B Cell-Dependent TCR Diversification

Cristina João, Brenda M. Ogle, Carlota Gay-Rabinstein, Jeffrey L. Platt, Marilia Cascalho

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

T cell diversity was once thought to depend on the interaction of T cell precursors with thymic epithelial cells. Recent evidence suggests, however, that diversity might arise through the interaction of developing T cells with other cells, the identity of which is not known. In this study we show that T cell diversity is driven by B cells and Ig. The TCR Vβ diversity of thymocytes in mice that lack B cells and Ig is reduced to 6 × 10 2 from wild-type values of 1.1 × 108; in mice with oligoclonal B cells, the TCR Vβ diversity of thymocytes is 0.01% that in wild-type mice. Adoptive transfer of diverse B cells or administration of polyclonal Ig increases thymocyte diversity in mice that lack B cells 8- and 7-fold, respectively, whereas adoptive transfer of monoclonal B cells or monoclonal Ig does not. These findings reveal a heretofore unrecognized and vital function of B cells and Ig for generation of T cell diversity and suggest a potential approach to immune reconstitution.

Original languageEnglish (US)
Pages (from-to)4709-4716
Number of pages8
JournalJournal of Immunology
Volume172
Issue number8
DOIs
StatePublished - Apr 15 2004
Externally publishedYes

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