Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin In Vivo

Thomas R. Cheever, Emily A. Olson, James M Ervasti

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The proper localization of ß-actin mRNA and protein is essential for growth cone guidance and axon elongation in cultured neurons. In addition, decreased levels of ß-actin mRNA and protein have been identified in the growth cones of motor neurons cultured from a mouse model of Spinal Muscular Atrophy (SMA), suggesting that ß-actin loss-of-function at growth cones or pre-synaptic nerve terminals could contribute to the pathogenesis of this disease. However, the role of ß-actin in motor neurons in vivo and its potential relevance to disease has yet to be examined. We therefore generated motor neuron specific ß-actin knock-out mice (Actb-MNsKO) to investigate the function of ß-actin in motor neurons in vivo. Surprisingly, ß-actin was not required for motor neuron viability or neuromuscular junction maintenance. Skeletal muscle from Actb-MNsKO mice showed no histological indication of denervation and did not significantly differ from controls in several measurements of physiologic function. Finally, motor axon regeneration was unimpaired in Actb-MNsKO mice, suggesting that ß-actin is not required for motor neuron function or regeneration in vivo.

Original languageEnglish (US)
Article numbere17768
JournalPloS one
Volume6
Issue number3
DOIs
StatePublished - Mar 28 2011

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Motor Neurons
motor neurons
Neurons
actin
Actins
Regeneration
Growth Cones
cones (retina)
Cones
axons
mice
Combustion knock
Spinal Muscular Atrophy
muscular atrophy
Messenger RNA
Neuromuscular Junction
Presynaptic Terminals
Denervation
Knockout Mice
Axons

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Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin In Vivo. / Cheever, Thomas R.; Olson, Emily A.; Ervasti, James M.

In: PloS one, Vol. 6, No. 3, e17768, 28.03.2011.

Research output: Contribution to journalArticle

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