Avian sarcoma virus envelope glycoprotein (gp85) specifically binds chick embryo fibroblasts

C. F. Moldow; F. H. Reynolds; John R Lake; K. Lundberg; J. R. Stephensonv

doi:10.1016/0042-6822(79)90354-4

Avian sarcoma virus envelope glycoprotein (gp85) specifically binds chick embryo fibroblasts

C. F. Moldow, F. H. Reynolds, John R Lake, K. Lundberg, J. R. Stephensonv

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Highly purified Prague (Pr) strain Rous sarcoma virus (RSV) subgroup C envelope glycoprotein (gp85) is shown to bind cellular receptor sites present on C/E, and to a much lesser extent C/C, chicken embryo fibroblasts. The specificity of this binding activity is indicated by the fact that neither C/E nor C/C chicken embryo fibroblasts exhibit detectable binding of Rauscher murine leukemia virus envelope glycoprotein (gp70). Moreover, of the avian viruses tested, only those with subgroup C host range inhibit binding of ¹²⁵I-labeled PrRSV-C gp85 by C/E chicken embryo fibroblasts. These findings provide a potential approach for quantitation and characterization of avian type C viral cellular receptor sites.

Original language	English (US)
Pages (from-to)	448-453
Number of pages	6
Journal	Virology
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(79)90354-4
State	Published - Sep 1979

Bibliographical note

Funding Information:
The authorst hank L. Crittenden of the East Lansing Regional Poultry Center for the gift of line 15b embryos. This work was supportedu nder Public Health Service Grant CA-13722a nd Contract NOI-CO-75336, National Cancer Institute, NIH, Bethesda, Maryland 20014.

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Copyright 2014 Elsevier B.V., All rights reserved.

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title = "Avian sarcoma virus envelope glycoprotein (gp85) specifically binds chick embryo fibroblasts",

abstract = "Highly purified Prague (Pr) strain Rous sarcoma virus (RSV) subgroup C envelope glycoprotein (gp85) is shown to bind cellular receptor sites present on C/E, and to a much lesser extent C/C, chicken embryo fibroblasts. The specificity of this binding activity is indicated by the fact that neither C/E nor C/C chicken embryo fibroblasts exhibit detectable binding of Rauscher murine leukemia virus envelope glycoprotein (gp70). Moreover, of the avian viruses tested, only those with subgroup C host range inhibit binding of 125I-labeled PrRSV-C gp85 by C/E chicken embryo fibroblasts. These findings provide a potential approach for quantitation and characterization of avian type C viral cellular receptor sites.",

author = "Moldow, {C. F.} and Reynolds, {F. H.} and Lake, {John R} and K. Lundberg and Stephensonv, {J. R.}",

note = "Funding Information: The authorst hank L. Crittenden of the East Lansing Regional Poultry Center for the gift of line 15b embryos. This work was supportedu nder Public Health Service Grant CA-13722a nd Contract NOI-CO-75336, National Cancer Institute, NIH, Bethesda, Maryland 20014. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1979",

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AU - Moldow, C. F.

AU - Reynolds, F. H.

AU - Lake, John R

AU - Lundberg, K.

AU - Stephensonv, J. R.

N1 - Funding Information: The authorst hank L. Crittenden of the East Lansing Regional Poultry Center for the gift of line 15b embryos. This work was supportedu nder Public Health Service Grant CA-13722a nd Contract NOI-CO-75336, National Cancer Institute, NIH, Bethesda, Maryland 20014. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1979/9

Y1 - 1979/9

N2 - Highly purified Prague (Pr) strain Rous sarcoma virus (RSV) subgroup C envelope glycoprotein (gp85) is shown to bind cellular receptor sites present on C/E, and to a much lesser extent C/C, chicken embryo fibroblasts. The specificity of this binding activity is indicated by the fact that neither C/E nor C/C chicken embryo fibroblasts exhibit detectable binding of Rauscher murine leukemia virus envelope glycoprotein (gp70). Moreover, of the avian viruses tested, only those with subgroup C host range inhibit binding of 125I-labeled PrRSV-C gp85 by C/E chicken embryo fibroblasts. These findings provide a potential approach for quantitation and characterization of avian type C viral cellular receptor sites.

AB - Highly purified Prague (Pr) strain Rous sarcoma virus (RSV) subgroup C envelope glycoprotein (gp85) is shown to bind cellular receptor sites present on C/E, and to a much lesser extent C/C, chicken embryo fibroblasts. The specificity of this binding activity is indicated by the fact that neither C/E nor C/C chicken embryo fibroblasts exhibit detectable binding of Rauscher murine leukemia virus envelope glycoprotein (gp70). Moreover, of the avian viruses tested, only those with subgroup C host range inhibit binding of 125I-labeled PrRSV-C gp85 by C/E chicken embryo fibroblasts. These findings provide a potential approach for quantitation and characterization of avian type C viral cellular receptor sites.

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Avian sarcoma virus envelope glycoprotein (gp85) specifically binds chick embryo fibroblasts

Abstract

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