Avian pneumovirus infection in Minnesota turkeys: Experimental reproduction of the disease

Faris F. Jirjis, Sally L. Noll, David A. Halvorson, Kakambi V. Nagaraja, Evelyn L. Townsend, Anwar M. Sheikh, Daniel P. Shaw

Research output: Contribution to journalArticle

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Abstract

Avian pneumovirus (APV) is an emerging viral respiratory disease agent of turkeys in Minnesota. Clinical signs of APV infection include open mouth breathing, ocular and nasal discharge, and swelling of infraorbital sinuses. The virus spreads rapidly among flocks of susceptible turkeys and is associated with increased mortality rates. A flock of 11-wk-old turkeys experienced a respiratory problem characterized by coughing, sneezing, swollen sinuses, and nasal discharge. The reverse transcriptase-polymerase chain reaction (RT-PCR) performed on tissues from the nasal turbinates and tracheal tissues was positive for avian pneumovirus. Turbinate tissue was inoculated into chicken embryo fibroblasts, and cytopathic effect was observed after five blind passages. In an attempt to reproduce the disease, 50 μl of this cell culture-propagated virus was instilled into each conjunctival space and nostril of 23-day-old turkey poults. The poults were sacrificed at 2-day intervals for 12 days, and serum, tissues, and tracheal and cloacal swabs were collected. Between days 2 and 10 after exposure, the poults developed ocular and nasal discharge and swollen sinuses. The virus was detected by RT-PCR and virus isolation from the nasal turbinates of poults sacrificed on days 4 and 6 postinoculation. Antibodies to APV were detected by enzyme-linked immunosorbent assay.

Original languageEnglish (US)
Pages (from-to)222-226
Number of pages5
JournalAvian Diseases
Volume44
Issue number1
StatePublished - Jan 2000

Fingerprint

Pneumovirus Infections
Metapneumovirus
Nose
Reproduction
Viruses
Avian metapneumovirus
poults
viruses
Turbinates
sinuses
Reverse Transcriptase Polymerase Chain Reaction
flocks
reverse transcriptase polymerase chain reaction
infection
Mouth Breathing
Sneezing
Paranasal Sinuses
Virus Diseases
Chickens
Embryonic Structures

Keywords

  • Avian pneumovirus
  • Respiratory disease
  • Turkeys

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Avian pneumovirus infection in Minnesota turkeys : Experimental reproduction of the disease. / Jirjis, Faris F.; Noll, Sally L.; Halvorson, David A.; Nagaraja, Kakambi V.; Townsend, Evelyn L.; Sheikh, Anwar M.; Shaw, Daniel P.

In: Avian Diseases, Vol. 44, No. 1, 01.2000, p. 222-226.

Research output: Contribution to journalArticle

Jirjis, Faris F.; Noll, Sally L.; Halvorson, David A.; Nagaraja, Kakambi V.; Townsend, Evelyn L.; Sheikh, Anwar M.; Shaw, Daniel P. / Avian pneumovirus infection in Minnesota turkeys : Experimental reproduction of the disease.

In: Avian Diseases, Vol. 44, No. 1, 01.2000, p. 222-226.

Research output: Contribution to journalArticle

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