Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins

William M. Gray, Stefan Kepinski, Dean Rouse, Ottoline Leyser, Mark Estelle

Research output: Contribution to journalArticlepeer-review

923 Scopus citations

Abstract

The plant hormone auxin is central in many aspects of plant development. Previous studies have implicated the ubiquitin-ligase SCFTIR1 and the AUX/IAA proteins in auxin response. Dominant mutations in several AUX/IAA genes confer pleiotropic auxin-related phenotypes, whereas recessive mutations affecting the function of SCFTIR1 decrease auxin response. Here we show that SCFTIR1 is required for AUX/IAA degradation. We demonstrate that SCFTIR1 interacts with AXR2/IAA7 and AXR3/IAA17, and that domain II of these proteins is necessary and sufficient for this interaction. Further, auxin stimulates binding of SCFTIR1 to the AUX/IAA proteins, and their degradation. Because domain II is conserved in nearly all AUX/IAA proteins in Arabidopsis, we propose that auxin promotes the degradation of this large family of transcriptional regulators, leading to diverse downstream effects.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalNature
Volume414
Issue number6861
DOIs
StatePublished - Nov 15 2001

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