Automated microdroplet platform for sample manipulation and polymerase chain reaction

Max Chabert, Kevin D. Dorfman, Patricia De Cremoux, Johan Roeraade, Jean Louis Viovy

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

We present a fully automated system performing continuous sampling, reagent mixing, and polymerase chain reaction (PCR) in microdroplets transported in immiscible oil. Sample preparation and analysis are totally automated, using an original injection method from a modified 96-well plate layered with three superimposed liquid layers and in-capillary laser-induced fluorescence endpoint detection. The process is continuous, allowing sample droplets to be carried uninterruptedly into the reaction zone while new drops are aspirated from the sample plate. Reproducible amplification, negligible cross-contamination, and detection of low sample concentrations were demonstrated on numerous consecutive sample drops. The system, which opens the route to strong reagents and labor savings in high-throughput applications, was validated on the clinically relevant quantification of progesterone receptor gene expression in human breast cancer cell lines.

Original languageEnglish (US)
Pages (from-to)7722-7728
Number of pages7
JournalAnalytical Chemistry
Volume78
Issue number22
DOIs
StatePublished - Nov 15 2006

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