Abstract
We present a fully automated system for performing continuous sampling, reagent mixing and Polymerase Chain Reaction (PCR) in microdroplets transported in immiscible oil. Reproducible amplification, negligible cross contamination and low detection limits were demonstrated in continuous operation on numerous consecutive sample drops. The system, which opens the route to strong reagents and labor savings in high throughput applications, was validated on the clinically relevant quantification of progesterone receptor gene expression in human breast cancer cell lines.
| Original language | English (US) |
|---|---|
| Title of host publication | Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference |
| Subtitle of host publication | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Japan Academic Association Inc |
| Pages | 1220-1222 |
| Number of pages | 3 |
| State | Published - Jan 1 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006 |
Other
| Other | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 11/5/06 → 11/9/06 |
Keywords
- Automation
- Continuous flow
- Droplets
- Interface
- PCR
