ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor

Rujie Cai, Ian R. Price, Fang Ding, Feifei Wu, Ting Chen, Yunlong Zhang, Guangfeng Liu, Paul J Jardine, Changrui Lu, Ailong Ke

Research output: Contribution to journalArticle

Abstract

Packaging of phage phi29 genome requires the ATPase gp16 and prohead RNA (pRNA). The highly conserved pRNA forms the interface between the connector complex and gp16. Understanding how pRNA interacts with gp16 under packaging conditions can shed light on the molecular mechanism of the packaging motor. Here, we present 3D models of the pRNA-gp16 complex and its conformation change in response to ATP or ADP binding. Using a combination of crystallography, small angle X-ray scattering and chemical probing, we find that the pRNA and gp16 forms a 'Z'-shaped complex, with gp16 specifically binds to pRNA domain II. The whole complex closes in the presence of ATP, and pRNA domain II rotates open as ATP hydrolyzes, before resetting after ADP is released. Our results suggest that pRNA domain II actively participates in the packaging process.

Original languageEnglish (US)
Pages (from-to)9818-9828
Number of pages11
JournalNucleic acids research
Volume47
Issue number18
DOIs
StatePublished - Oct 10 2019

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DNA Packaging
Adenosine Diphosphate
Product Packaging
Adenosine Triphosphate
RNA
Crystallography
Bacteriophages
Adenosine Triphosphatases
X-Rays
Genome
RNA primers

PubMed: MeSH publication types

  • Journal Article

Cite this

Cai, R., Price, I. R., Ding, F., Wu, F., Chen, T., Zhang, Y., ... Ke, A. (2019). ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor. Nucleic acids research, 47(18), 9818-9828. https://doi.org/10.1093/nar/gkz692

ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor. / Cai, Rujie; Price, Ian R.; Ding, Fang; Wu, Feifei; Chen, Ting; Zhang, Yunlong; Liu, Guangfeng; Jardine, Paul J; Lu, Changrui; Ke, Ailong.

In: Nucleic acids research, Vol. 47, No. 18, 10.10.2019, p. 9818-9828.

Research output: Contribution to journalArticle

Cai, R, Price, IR, Ding, F, Wu, F, Chen, T, Zhang, Y, Liu, G, Jardine, PJ, Lu, C & Ke, A 2019, 'ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor', Nucleic acids research, vol. 47, no. 18, pp. 9818-9828. https://doi.org/10.1093/nar/gkz692
Cai, Rujie ; Price, Ian R. ; Ding, Fang ; Wu, Feifei ; Chen, Ting ; Zhang, Yunlong ; Liu, Guangfeng ; Jardine, Paul J ; Lu, Changrui ; Ke, Ailong. / ATP/ADP modulates gp16-pRNA conformational change in the Phi29 DNA packaging motor. In: Nucleic acids research. 2019 ; Vol. 47, No. 18. pp. 9818-9828.
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AU - Jardine, Paul J

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