TY - JOUR
T1 - ATP-dependent proton transport by isolated brain clathrin-coated vesicles. Role of clathrin and other determinants of acidification
AU - Van Dyke, Rebecca W.
AU - Scharschmidt, Bruce F.
AU - Steer, Clifford J.
PY - 1985/1/25
Y1 - 1985/1/25
N2 - We have systematically investigated certain characteristics of the ATP-dependent proton transport mechanism of bovine brain clathrin-coated vesicles. H+ transport specific activity was shown by column chromatography to co-purify with coated vesicles, however, the clathrin coat is not required for vesicle acidification as H+ transport was not altered by prior removal of the clathrin coat. Acidification of the vesicle interior, measured by fluorescence quenching of acridine orange, displayed considerable anion selectively (CI- > Br-≫NO3-≫ gluconate, SO42-, HPO42-, mannitol; Km for Cl- ≈- 15 mM), but was relatively insensitive to cation replacement as long as Cl- was present. Acidification was unaffected by ouabain or vanadate but was inhibited by N-ethylmaleimide (IC50 < 10 μM), dicyclohexylcarbodiimide (DCCD) (lC50 ≈- 10 μM), chlorpromazine (lC50 ≈- 15 μM), and oligomycin (lC50 ≈- 3 μM). In contrast to N-ethylmaleimide, chlorpromazine rapidly dissipated preformed pH gradients. Valinomycin stimulated H+ transport in the presence of potassium salts (gluconate ≫ NO3- > Cl-), and the membrane-potential-sensitive dye Oxonol V demonstrated an ATP-dependent interior-positive vesicle membrane potential which was greater in the absence of permeant anions (mannitol > potassium gluconate > KCI) and was abolished by N-ethylmaleimide, protonophores or detergent. Total vesicle-associated ouabain-insensitive ATPase activity was inhibited 64% by 1 mM N-ethylmaleimide, and correlated poorly with H+ transport, however N-ethylmaleimide-sensitive ATPase activity correlated well with proton transport (r = 0.95) in the presence of various Cl- salts and KNO3- Finally, vesicles prepared from bovine brain synaptic membranes exhibited H+ transport activity similar to that of the coated vesicles. Collectively these findings indicate that: (1) the H+ transport mechanism of bovine brain clathrin-coated vesicles is not dependent upon the clathrin coat, and closely resembles that of rat liver clathrin-coated vesicles, (2) the H+ transport mechanism is intrinsically electrogenic and pH and electrical gradients established by the proton transport mechanism vary inversely in the presence of permeable or impermeable anions, (3) at concentrations above 3 μM, oligomycin inhibits vesicle acidification, (4) chlorpromazine inhibits coated vesicle acidification, at least in part, by increasing proton conductance, and (5) other smooth membranes from bovine brain, including synaptic membranes, exhibit a similar H+ transport mechanism.
AB - We have systematically investigated certain characteristics of the ATP-dependent proton transport mechanism of bovine brain clathrin-coated vesicles. H+ transport specific activity was shown by column chromatography to co-purify with coated vesicles, however, the clathrin coat is not required for vesicle acidification as H+ transport was not altered by prior removal of the clathrin coat. Acidification of the vesicle interior, measured by fluorescence quenching of acridine orange, displayed considerable anion selectively (CI- > Br-≫NO3-≫ gluconate, SO42-, HPO42-, mannitol; Km for Cl- ≈- 15 mM), but was relatively insensitive to cation replacement as long as Cl- was present. Acidification was unaffected by ouabain or vanadate but was inhibited by N-ethylmaleimide (IC50 < 10 μM), dicyclohexylcarbodiimide (DCCD) (lC50 ≈- 10 μM), chlorpromazine (lC50 ≈- 15 μM), and oligomycin (lC50 ≈- 3 μM). In contrast to N-ethylmaleimide, chlorpromazine rapidly dissipated preformed pH gradients. Valinomycin stimulated H+ transport in the presence of potassium salts (gluconate ≫ NO3- > Cl-), and the membrane-potential-sensitive dye Oxonol V demonstrated an ATP-dependent interior-positive vesicle membrane potential which was greater in the absence of permeant anions (mannitol > potassium gluconate > KCI) and was abolished by N-ethylmaleimide, protonophores or detergent. Total vesicle-associated ouabain-insensitive ATPase activity was inhibited 64% by 1 mM N-ethylmaleimide, and correlated poorly with H+ transport, however N-ethylmaleimide-sensitive ATPase activity correlated well with proton transport (r = 0.95) in the presence of various Cl- salts and KNO3- Finally, vesicles prepared from bovine brain synaptic membranes exhibited H+ transport activity similar to that of the coated vesicles. Collectively these findings indicate that: (1) the H+ transport mechanism of bovine brain clathrin-coated vesicles is not dependent upon the clathrin coat, and closely resembles that of rat liver clathrin-coated vesicles, (2) the H+ transport mechanism is intrinsically electrogenic and pH and electrical gradients established by the proton transport mechanism vary inversely in the presence of permeable or impermeable anions, (3) at concentrations above 3 μM, oligomycin inhibits vesicle acidification, (4) chlorpromazine inhibits coated vesicle acidification, at least in part, by increasing proton conductance, and (5) other smooth membranes from bovine brain, including synaptic membranes, exhibit a similar H+ transport mechanism.
KW - (Brain vesicle)
KW - ATP dependence
KW - Acidification
KW - Clathrin
KW - Proton transport
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UR - http://www.scopus.com/inward/citedby.url?scp=0021909658&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(85)90317-7
DO - 10.1016/0005-2736(85)90317-7
M3 - Article
C2 - 2857093
AN - SCOPUS:0021909658
VL - 812
SP - 423
EP - 436
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -