Atomic-level mechanisms for phospholamban regulation of the calcium pump

L. Michel Espinoza-Fonseca, Joseph M. Autry, G. Lizbeth Ramírez-Salinas, David D. Thomas

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We performed protein pKa calculations and molecular dynamics (MD) simulations of the calcium pump (sarcoplasmic reticulum Ca2+-ATPase (SERCA)) in complex with phospholamban (PLB). X-ray crystallography studies have suggested that PLB locks SERCA in a low-Ca2+-affinity E2 state that is incompatible with metal-ion binding, thereby blocking the conversion toward a high-Ca2+-affinity E1 state. Estimation of pKa values of the acidic residues in the transport sites indicates that at normal intracellular pH (7.1-7.2), PLB-bound SERCA populates an E1 state that is deprotonated at residues E309 and D800 yet protonated at residue E771. We performed three independent microsecond-long MD simulations to evaluate the structural dynamics of SERCA-PLB in a solution containing 100 mM K+ and 3 mM Mg2+. Principal component analysis showed that PLB-bound SERCA lies exclusively along the structural ensemble of the E1 state. We found that the transport sites of PLB-bound SERCA are completely exposed to the cytosol and that K+ ions bind transiently (≤5 ns) and nonspecifically (nine different positions) to the two transport sites, with a total occupancy time of K;bsupesup& in the transport sites of 80%. We propose that PLB binding to SERCA populates a novel (to our knowledge) E1 intermediate, E1·H+771. This intermediate serves as a kinetic trap that controls headpiece dynamics and depresses the structural transitions necessary for Ca2+-dependent activation of SERCA. We conclude that PLB-mediated regulation of SERCA activity in the heart results from biochemical and structural transitions that occur primarily in the E1 state of the pump.

Original languageEnglish (US)
Pages (from-to)1697-1708
Number of pages12
JournalBiophysical journal
Volume108
Issue number7
DOIs
StatePublished - Apr 7 2015

Bibliographical note

Funding Information:
This work was supported by grants from the American Heart Association (12SDG12060656 to L.M.E.-F.) and the National Institutes of Health (R01GM27906 to D.D.T.). G.L.R.-S. is supported by a predoctoral fellowship from CONACYT (Mexico). This project made extensive use of the outstanding facilities at the University of Minnesota Supercomputing Institute.

Publisher Copyright:
© 2015 Biophysical Society.

Fingerprint

Dive into the research topics of 'Atomic-level mechanisms for phospholamban regulation of the calcium pump'. Together they form a unique fingerprint.

Cite this