Atg1-independent induction of autophagy by the Drosophila Ulk3 homolog, ADUK

Christopher R. Braden, Thomas P. Neufeld

Research output: Contribution to journalEditorial

5 Scopus citations

Abstract

Although canonical autophagy regulation requires a multi-protein complex centered on the Ser/Thr-kinase Atg1 (mammalian Ulk1/2), alternative signals can induce autophagy independent of Atg1 through unknown mechanisms. Here we identify the Drosophila Ulk3 ortholog, another Drosophila Unc-51-like kinase (ADUK), as an Atg1-independent autophagy inducer. ADUK interacts with Atg1 complex members Atg13 and 200 kDa FAK family kinase-interacting protein, and requires Atg13 but not Atg1 for autophagy induction. Loss of ADUK shortens adult lifespan and reduces the autophagic response to a chemical stressor, dimethyl sulfoxide. However, ADUK is not required for autophagy induction by Atg1-dependent nutrient or developmental cues. Atg1 and ADUK/Ulk3 thus represent alternative catalytic components of a shared autophagy kinase complex.

Original languageEnglish (US)
Pages (from-to)3889-3897
Number of pages9
JournalFEBS Journal
Volume283
Issue number21
DOIs
StatePublished - Nov 1 2016

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Keywords

  • Atg1
  • Drosophila
  • Ulk1
  • Ulk3
  • autophagy

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