AtCHX13 is a plasma membrane K+ transporter

Jian Zhao, Ning Hui Cheng, Christy M. Motes, Elison B. Blancaflor, Miranda Moore, Naomi Gonzales, Senthilkumar Padmanaban, Heven Sze, John M. Ward, Kendal D. Hirschi

Research output: Contribution to journalArticle

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Abstract

Potassium (K+) homeostasis is essential for diverse cellular processes, although how various cation transporters collaborate to maintain a suitable K+ required for growth and development is poorly understood. The Arabidopsis (Arabidopsis thaliana) genome contains numerous cation:proton antiporters (CHX), which may mediate K+ transport; however, the vast majority of these transporters remain uncharacterized. Here, we show that AtCHX13 (At2g30240) has a role in K+ acquisition. AtCHX13 suppressed the sensitivity of yeast (Saccharomyces cerevisiae) mutant cells defective in K+ uptake. Uptake experiments using 86Rb+ as a tracer for K+ demonstrated that AtCHX13 mediated high-affinity K + uptake in yeast and in plant cells with a Km of 136 and 196 μM, respectively. Functional green fluorescent protein-tagged versions localized to the plasma membrane of both yeast and plant. Seedlings of null chx13 mutants were sensitive to K+ deficiency conditions, whereas overexpression of AtCHX13 reduced the sensitivity to K+ deficiency. Collectively, these results suggest that AtCHX13 mediates relatively high-affinity K+ uptake, although the mode of transport is unclear at present. AtCHX13 expression is induced in roots during K+-deficient conditions. These results indicate that one role of AtCHX13 is to promote K + uptake into plants when K+ is limiting in the environment.

Original languageEnglish (US)
Pages (from-to)796-807
Number of pages12
JournalPlant physiology
Volume148
Issue number2
DOIs
StatePublished - Oct 2008

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Membrane Transport Proteins
transporters
plasma membrane
Yeasts
Cell Membrane
uptake mechanisms
Arabidopsis
Cations
Antiporters
yeasts
Plant Cells
Green Fluorescent Proteins
Seedlings
Growth and Development
Saccharomyces cerevisiae
Protons
Potassium
Homeostasis
mutants
antiporters

Cite this

Zhao, J., Cheng, N. H., Motes, C. M., Blancaflor, E. B., Moore, M., Gonzales, N., ... Hirschi, K. D. (2008). AtCHX13 is a plasma membrane K+ transporter. Plant physiology, 148(2), 796-807. https://doi.org/10.1104/pp.108.124248

AtCHX13 is a plasma membrane K+ transporter. / Zhao, Jian; Cheng, Ning Hui; Motes, Christy M.; Blancaflor, Elison B.; Moore, Miranda; Gonzales, Naomi; Padmanaban, Senthilkumar; Sze, Heven; Ward, John M.; Hirschi, Kendal D.

In: Plant physiology, Vol. 148, No. 2, 10.2008, p. 796-807.

Research output: Contribution to journalArticle

Zhao, J, Cheng, NH, Motes, CM, Blancaflor, EB, Moore, M, Gonzales, N, Padmanaban, S, Sze, H, Ward, JM & Hirschi, KD 2008, 'AtCHX13 is a plasma membrane K+ transporter', Plant physiology, vol. 148, no. 2, pp. 796-807. https://doi.org/10.1104/pp.108.124248
Zhao J, Cheng NH, Motes CM, Blancaflor EB, Moore M, Gonzales N et al. AtCHX13 is a plasma membrane K+ transporter. Plant physiology. 2008 Oct;148(2):796-807. https://doi.org/10.1104/pp.108.124248
Zhao, Jian ; Cheng, Ning Hui ; Motes, Christy M. ; Blancaflor, Elison B. ; Moore, Miranda ; Gonzales, Naomi ; Padmanaban, Senthilkumar ; Sze, Heven ; Ward, John M. ; Hirschi, Kendal D. / AtCHX13 is a plasma membrane K+ transporter. In: Plant physiology. 2008 ; Vol. 148, No. 2. pp. 796-807.
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