Astragalus polysaccharide improves palmitate-induced insulin resistance by inhibiting PTP1B and NF-κB in C2C12 myotubes

Ming Zhao, Zhao Feng Zhang, Ye Ding, Jun Bo Wang, Yong Li

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

We investigated the effects of Astragalus polysaccharide (APS) on palmitate-induced insulin resistance in C2C12 skeletal muscle myotubes. Palmitate-reduced glucose uptake was restored by APS. APS prevented palmitate-induced C2C12 myotubes from impaired insulin signaling by inhibiting Ser307 phosphorylation of insulin receptor substrate-1 (IRS-1) and increasing Ser473 phosphorylation of Akt. Moreover, the increases in protein-tyrosine phosphatase-1B (PTP1B) protein level and NF-κB activation associated with palmitate treatment were also prevented by APS. However the treatment with APS didn't change AMP-activated protein kinase (AMPK) activation in palmitate-induced myotubes. The results of the present study suggest that Astragalus polysaccharide inhibits palmitate-induced insulin resistance in C2C12 myotubes by inhibiting expression of PTP1B and regulating NF-κB but not AMPK pathway.

Original languageEnglish (US)
Pages (from-to)7083-7092
Number of pages10
JournalMolecules
Volume17
Issue number6
DOIs
StatePublished - Jun 2012

Keywords

  • Astragalus polysaccharide
  • Insulin resistance
  • NF-κB
  • PTP1B

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