Red-shanked doucs (Pygathrix nemaeus) are endangered, foregut-fermenting colobine primates which are difficult to maintain in captivity. There are critical gaps in our understanding of their natural lifestyle, including dietary habits such as consumption of leaves, unripe fruit, flowers, seeds, and other plant parts. There is also a lack of understanding of enteric adaptations, including their unique microflora. To address these knowledge gaps, we used the douc as a model to study relationships between gastrointestinal microbial community structure and lifestyle. We analyzed published fecal samples as well as detailed dietary history from doucs with four distinct lifestyles (wild, semi-wild, semi-captive, and captive) and determined gastrointestinal bacterial microbiome composition using 16S rRNA sequencing. A clear gradient of microbiome composition was revealed along an axis of natural lifestyle disruption, including significant associations with diet, biodiversity, and microbial function. We also identified potential microbial biomarkers of douc dysbiosis, including Bacteroides and Prevotella, which may be related to health. Our results suggest a gradient-like shift in captivity causes an attendant shift to severe gut dysbiosis, thereby resulting in gastrointestinal issues.
|Original language||English (US)|
|State||Published - Dec 1 2018|
Bibliographical noteFunding Information:
We thank the Endangered Primate Rescue Center, Singapore Zoo, and Philadelphia Zoo for providing fecal samples from semi-wild, semi-captive, and captive red-shanked doucs; Tran Van Luong, Nguyen Van Bay, and Nguyen Manh Tien for their permission to work in Son Tra Nature Reserve and for their continued support and help; Kieu Thi Kinh and Thai Van Quang for help in obtaining the research permits; the Department of Forest Protection, the Da Nang University, and the Son Tra Nature Reserve for granting the research permits; and Christina Valeri and James Collins at the University of Minnesota Veterinary Diagnostic Laboratory for their assistance with acquiring and maintaining shipping permits. This research was funded in part by the Margot Marsh Biodiversity Foundation; the Mohamed bin Zayed Species Conservation Fund; and the National Institutes of Health through a PharmacoNeuroImmunology Fellowship (NIH/National Institute on Drug Abuse T32 DA007097-32) awarded to JBC.
© 2018, The Author(s).