Abstract
The frequent use of insecticides to manage soybean aphids, Aphis glycines (Hemiptera: Aphididae), in the United States has contributed to field-evolved resistance. Pyrethroid-resistant aphids have nonsynonymous mutations in the voltage-gated sodium channel (vgsc). We identified a leucine to phenylalanine mutation at position 1014 (L1014F) and a methionine to isoleucine mutation (M918I) of the A. glycines vgsc, both suspected of conferring knockdown resistance (kdr) to lambda-cyhalothrin. We developed molecular markers to identify these mutations in insecticide-resistant aphids. We determined that A. glycines which survived exposure to a diagnostic concentration of lambda-cyhalothrin and bifenthrin via glass-vial bioassays had these mutations, and showed significant changes in the resistance allele frequency between samples collected before and after field application of lambda-cyhalothrin. Thus, a strong association was revealed between aphids with L1014F and M918I vgsc mutations and survival following exposure to pyrethroids. Specifically, the highest survival was observed for aphids with the kdr (L1014F) and heterozygote super-kdr (L1014F + M918I) genotypes following laboratory bioassays and in-field application of lambda-cyhalothrin. These genetic markers could be used as a diagnostic tool for detecting insecticide-resistant A. glycines and monitoring the geographic distribution of pyrethroid resistance. We discuss how generating these types of data could improve our efforts to mitigate the effects of pyrethroid resistance on crop production.
| Original language | English (US) |
|---|---|
| Article number | 12020 |
| Journal | Scientific reports |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2022 |
Bibliographical note
Funding Information:This work was funded by soybean checkoff funds from the Iowa Soybean Association and a United States Department of Agriculture (USDA) National Institute for Food and Agriculture, Crop Protection and Pest Management (Grant #: 2020-70006-33018). This work was also supported and funded by the USDA, Agricultural Research Service (ARS) (CRIS Project 5030-22000-019-00D). This article reports the results of research only, and mention of any commercial product or service does not constitute a recommendation for its use by USDA. USDA is an equal opportunity employer and provider. The authors would like to thank Greg VanNostrand, Ashley Dean, Kelsey Shepherd, and Matthew Weiser for technical assistance in collecting and processing aphid samples, and Tina Ward for laboratory support.
Publisher Copyright:
© 2022, The Author(s).
