Abstract
Natural occurrence of yellow mosaic disease was observed on Armenian cucumber (Cucumis melo var. flexuoses) and wild melon (C. callosus var. agrestis) with disease incidences of ~36 and ~27%, respectively. Association of tomato leaf curl Palampur virus (ToLCPV) with the disease was investigated by Polymersae chain reaction (PCR) using begomovirus-specific primers. Full-length genome was amplified by rolling circle amplification (RCA) method from representative samples of C. melo and C. callosus. RCA products obtained were cloned and sequenced. Analyses of sequence data revealed the presence of full-length begomoviral genome of 2756 nucleotides with the gene arrangement of a typical begomovirus: HQ848383 (C. melo) and GU253914 (C. callosus). Both the isolates shared 99% sequence identity together and high 97–99% identities and the closest phylogenetic relationships with ToLCPV strains reported worldwide, hence identified as two new members of ToLCPV. Natural occurrence of ToLCPV on C. melo and C. callosus is the first report.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 751-759 |
| Number of pages | 9 |
| Journal | Archives of Phytopathology and Plant Protection |
| Volume | 48 |
| Issue number | 9-12 |
| DOIs | |
| State | Published - Jul 21 2015 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2016 Taylor & Francis.
Keywords
- Armenian cucumber
- begomovirus
- sequence analyses
- tomato leaf curl Palampur virus
- wild melon
- yellow mosaic disease