Association of low-dose exposure to persistent organic pollutants with global DNA hypomethylation in healthy Koreans

Keon Yeop Kim, Dong Sun Kim, Sung Kook Lee, In Kyu Lee, Jung Ho Kang, Yoon Seok Chang, David R Jacobs Jr, Michael W Steffes, Duk Hee Lee

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

BACKGROUND: Global DNA methylation levels have been reported to be inversely associated with blood levels of persistent organic pollutants (POPs), xenobiotics that accumulate in adipose tissue. Whether these associations extend to a population with much lower concentrations of POPs is not known. OBJECTIVES: This study was performed to examine whether low-dose exposure to POPs was associated with global DNA hypomethylation in Koreans. METHODS: The amount of global DNA hypomethylation was estimated by the percent 5-methylcytosine (%5-mC) in Alu and LINE-1 assays in 86 apparently healthy Koreans. Among various POPs, organochlorine (OC) pesticides, polychlorinated biphenyls (PCBs), and polybrominated diphenylethers (PBDEs) were measured. RESULTS: Most OC pesticides were inversely and significantly associated with %5-mC in the Alu assay, with correlation coefficients in the range -0.2 to -0.3 after adjusting for age, sex, body mass index, smoking, and alcohol. The strongest OC pesticide associations with %5-mC in the Alu assay were observed with oxychlordane, trans-nonachlor, and p,ṕ- dichlorodiphenyldichloroethylene. The correlation coefficient of age with %5-mC in the Alu assay was -0.24, similar to correlations of OC pesticides with %5-mC in the Alu assay. Most PCBs and PBDEs showed nonsignificant inverse trends with %5-mC in the Alu assay, but for some PCBs the U-shaped association was significant. On the other hand, POPs were not associated with %5-mC in the LINE-1 assay. CONCLUSIONS: We found that low-dose exposure to POPs, in particular OC pesticides, was associated with global DNA hypomethylation in apparently healthy Koreans.

Original languageEnglish (US)
Pages (from-to)370-374
Number of pages5
JournalEnvironmental health perspectives
Volume118
Issue number3
DOIs
StatePublished - Mar 1 2010

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Pesticides
Polychlorinated Biphenyls
DNA
5-Methylcytosine
Xenobiotics
DNA Methylation
Adipose Tissue
Body Mass Index
Smoking
Alcohols
Population

Keywords

  • Epigenetics
  • Hypomethylation
  • Organochlorine pesticides
  • Persistent organic pollutants

Cite this

Association of low-dose exposure to persistent organic pollutants with global DNA hypomethylation in healthy Koreans. / Kim, Keon Yeop; Kim, Dong Sun; Lee, Sung Kook; Lee, In Kyu; Kang, Jung Ho; Chang, Yoon Seok; Jacobs Jr, David R; Steffes, Michael W; Lee, Duk Hee.

In: Environmental health perspectives, Vol. 118, No. 3, 01.03.2010, p. 370-374.

Research output: Contribution to journalArticle

Kim, Keon Yeop ; Kim, Dong Sun ; Lee, Sung Kook ; Lee, In Kyu ; Kang, Jung Ho ; Chang, Yoon Seok ; Jacobs Jr, David R ; Steffes, Michael W ; Lee, Duk Hee. / Association of low-dose exposure to persistent organic pollutants with global DNA hypomethylation in healthy Koreans. In: Environmental health perspectives. 2010 ; Vol. 118, No. 3. pp. 370-374.
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AU - Kim, Keon Yeop

AU - Kim, Dong Sun

AU - Lee, Sung Kook

AU - Lee, In Kyu

AU - Kang, Jung Ho

AU - Chang, Yoon Seok

AU - Jacobs Jr, David R

AU - Steffes, Michael W

AU - Lee, Duk Hee

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N2 - BACKGROUND: Global DNA methylation levels have been reported to be inversely associated with blood levels of persistent organic pollutants (POPs), xenobiotics that accumulate in adipose tissue. Whether these associations extend to a population with much lower concentrations of POPs is not known. OBJECTIVES: This study was performed to examine whether low-dose exposure to POPs was associated with global DNA hypomethylation in Koreans. METHODS: The amount of global DNA hypomethylation was estimated by the percent 5-methylcytosine (%5-mC) in Alu and LINE-1 assays in 86 apparently healthy Koreans. Among various POPs, organochlorine (OC) pesticides, polychlorinated biphenyls (PCBs), and polybrominated diphenylethers (PBDEs) were measured. RESULTS: Most OC pesticides were inversely and significantly associated with %5-mC in the Alu assay, with correlation coefficients in the range -0.2 to -0.3 after adjusting for age, sex, body mass index, smoking, and alcohol. The strongest OC pesticide associations with %5-mC in the Alu assay were observed with oxychlordane, trans-nonachlor, and p,ṕ- dichlorodiphenyldichloroethylene. The correlation coefficient of age with %5-mC in the Alu assay was -0.24, similar to correlations of OC pesticides with %5-mC in the Alu assay. Most PCBs and PBDEs showed nonsignificant inverse trends with %5-mC in the Alu assay, but for some PCBs the U-shaped association was significant. On the other hand, POPs were not associated with %5-mC in the LINE-1 assay. CONCLUSIONS: We found that low-dose exposure to POPs, in particular OC pesticides, was associated with global DNA hypomethylation in apparently healthy Koreans.

AB - BACKGROUND: Global DNA methylation levels have been reported to be inversely associated with blood levels of persistent organic pollutants (POPs), xenobiotics that accumulate in adipose tissue. Whether these associations extend to a population with much lower concentrations of POPs is not known. OBJECTIVES: This study was performed to examine whether low-dose exposure to POPs was associated with global DNA hypomethylation in Koreans. METHODS: The amount of global DNA hypomethylation was estimated by the percent 5-methylcytosine (%5-mC) in Alu and LINE-1 assays in 86 apparently healthy Koreans. Among various POPs, organochlorine (OC) pesticides, polychlorinated biphenyls (PCBs), and polybrominated diphenylethers (PBDEs) were measured. RESULTS: Most OC pesticides were inversely and significantly associated with %5-mC in the Alu assay, with correlation coefficients in the range -0.2 to -0.3 after adjusting for age, sex, body mass index, smoking, and alcohol. The strongest OC pesticide associations with %5-mC in the Alu assay were observed with oxychlordane, trans-nonachlor, and p,ṕ- dichlorodiphenyldichloroethylene. The correlation coefficient of age with %5-mC in the Alu assay was -0.24, similar to correlations of OC pesticides with %5-mC in the Alu assay. Most PCBs and PBDEs showed nonsignificant inverse trends with %5-mC in the Alu assay, but for some PCBs the U-shaped association was significant. On the other hand, POPs were not associated with %5-mC in the LINE-1 assay. CONCLUSIONS: We found that low-dose exposure to POPs, in particular OC pesticides, was associated with global DNA hypomethylation in apparently healthy Koreans.

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KW - Persistent organic pollutants

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