Association of genetic variants of RANK, RANKL, and OPG with ankylosing spondylitis clinical features in Taiwanese

Chin Man Wang, Shu Chun Tsai, Jing Chi Lin, Yeong Jian Jan Wu, Jianming Wu, Ji Yih Chen

Research output: Contribution to journalArticle

Abstract

Ankylosing spondylitis (AS) is a chronic inflammatory disease that leads to spinal ankylosis. The receptor activator of the nuclear factor-kappa (RANK), RANK ligand, and osteoprotegerin (OPG) (RANK/RANKL/OPG) pathway plays critical roles in bone metabolism and the immune system. The current study was aimed at investigating whether six single-nucleotide polymorphisms (SNPs) within the RANK, RANKL, and OPG genes essential for bone homeostasis are associated with AS. Genotype distributions, allele and haplotype frequencies, were compared between 1120 AS patients and 1435 healthy controls and among AS patients with stratification by syndesmophyte formation, onset age, and HLA-B27 positivity. We found that RANKL SNPs were associated with AS syndesmophyte formation. Notably, the RANKL SNP haplotype rs7984870C/rs9533155G/rs9525641C was negatively associated with AS susceptibility and appeared to protect against syndesmophyte formation in AS. Functionally, RANKL promoter SNPs (rs9525641 C/T and rs9533155 G/C) affected DNA-protein complex formation and promoter activity in promoter reporter analyses. The OPG SNP haplotype rs2073618G/rs3102735T was significantly associated with HLA-B27 negativity in AS patients. Furthermore, AS patients with syndesmophyte formation had significantly lower levels of soluble RANKL levels than those without syndesmophyte formation. Our data suggested a role for RANKL in AS susceptibility and severity.

Original languageEnglish (US)
Article number8029863
JournalMediators of inflammation
Volume2019
DOIs
StatePublished - Jan 1 2019

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Osteoprotegerin
Ankylosing Spondylitis
Cytoplasmic and Nuclear Receptors
Single Nucleotide Polymorphism
Haplotypes
HLA-B27 Antigen
RANK Ligand
Ankylosis
Bone and Bones
kappa Opioid Receptor
Critical Pathways
Essential Genes
Age of Onset
Gene Frequency
Immune System
Homeostasis
Chronic Disease
Genotype

PubMed: MeSH publication types

  • Journal Article

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Association of genetic variants of RANK, RANKL, and OPG with ankylosing spondylitis clinical features in Taiwanese. / Wang, Chin Man; Tsai, Shu Chun; Lin, Jing Chi; Wu, Yeong Jian Jan; Wu, Jianming; Chen, Ji Yih.

In: Mediators of inflammation, Vol. 2019, 8029863, 01.01.2019.

Research output: Contribution to journalArticle

Wang, Chin Man ; Tsai, Shu Chun ; Lin, Jing Chi ; Wu, Yeong Jian Jan ; Wu, Jianming ; Chen, Ji Yih. / Association of genetic variants of RANK, RANKL, and OPG with ankylosing spondylitis clinical features in Taiwanese. In: Mediators of inflammation. 2019 ; Vol. 2019.
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abstract = "Ankylosing spondylitis (AS) is a chronic inflammatory disease that leads to spinal ankylosis. The receptor activator of the nuclear factor-kappa (RANK), RANK ligand, and osteoprotegerin (OPG) (RANK/RANKL/OPG) pathway plays critical roles in bone metabolism and the immune system. The current study was aimed at investigating whether six single-nucleotide polymorphisms (SNPs) within the RANK, RANKL, and OPG genes essential for bone homeostasis are associated with AS. Genotype distributions, allele and haplotype frequencies, were compared between 1120 AS patients and 1435 healthy controls and among AS patients with stratification by syndesmophyte formation, onset age, and HLA-B27 positivity. We found that RANKL SNPs were associated with AS syndesmophyte formation. Notably, the RANKL SNP haplotype rs7984870C/rs9533155G/rs9525641C was negatively associated with AS susceptibility and appeared to protect against syndesmophyte formation in AS. Functionally, RANKL promoter SNPs (rs9525641 C/T and rs9533155 G/C) affected DNA-protein complex formation and promoter activity in promoter reporter analyses. The OPG SNP haplotype rs2073618G/rs3102735T was significantly associated with HLA-B27 negativity in AS patients. Furthermore, AS patients with syndesmophyte formation had significantly lower levels of soluble RANKL levels than those without syndesmophyte formation. Our data suggested a role for RANKL in AS susceptibility and severity.",
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