Association of endothelial nitric oxide synthase gene polymorphism with level of uric acid in serum for acute coronary syndrome

Objective: To evaluate the association of elevation in serum uric acid with the development of coronary artery disease, and to determine the relationship between uric acid and Glu²⁹⁸ → Asp polymorphism of the endothelial nitric oxide synthase (eNOS) gene in acute coronary syndrome (ACS) in the Chinese Han Nationality. Methods: The Glu²⁹⁸→Asp variant of the eNOS gene was detected by polymerase chain reaction/restriction fragment length polymorphism analysis in 58 patients with ACS and 43 healthy controls. The severity of ACS was expressed by the number of affected vessels and by the Duke scoring system. Results: The frequencies of the eNOS Glu/Glu, Glu/Asp, and Asp/Asp genotypes in the ACS group were not significantly different from those of controls (43.1%, 36.2%, 20.7% vs. 48.8%, 34.9%, 16.3%, respectively; χ²=0.446, P=0.800). In comparison with subjects who had Glu²⁹⁸ allele in the eNOS gene, the risk of ACS was not increased among Asp/Asp carriers (odds ratio 1.34,95% confidence interval 0.479 to 3.755, P=0.575). There was no significant association between the eNOS Glu²⁹⁸→Asp variant and the Duke score [(46.73±19.90) score for Asp/ Asp vs. (48.33±19.61) score and (38.19±15.12) score for Glu/Glu and Glu/Asp, respectively, P=0.248], but there was a significant association between the eNOS Glu²⁹⁸→Asp variant and the serum uric acid level in ACS group [(298.92±87.27) μmol/L for Glu/Glu vs. (380.80 ± 95.80) μmol/L and (346.16 ± 93.71) μmol/L for Glu/Asp and Asp/Asp, respectively, P=0.017). Conclusion: Glu²⁹⁸→Asp polymorphism of the eNOS gene appears to have no association with ACS in the Chinese Han Nationality, but a significant association between the eNOS Glu²⁹⁸→Asp variant and the serum uric acid level is found in patients with ACS.