Association of β1 integrin with protein kinase activity in large detergent resistant complexes

Amy P Skubitz, Kenneth D. Campbell, Said Goueli, Keith M Skubitz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Integrins play a critical role in cell adhesion and mediate cell signaling. This report identifies the association of serine protein kinase activity with the β1 integrin by immunoprecipitation and phosphoamino acid analysis techniques. Reprecipitation techniques suggested that the serine kinase activity was not a member of the protein kinase C family. By gel filtration, most of the protein kinase activity associated with β1 integrin as well as most of the cell-surface β1 integrin was present in large detergent resistant complexes. These results suggest that serine protein kinase activity associated with the β1 integrin may play a role in signaling via the β1 integrin.

Original languageEnglish (US)
Pages (from-to)386-391
Number of pages6
JournalFEBS Letters
Volume426
Issue number3
DOIs
StatePublished - Apr 24 1998

Fingerprint

Integrins
Detergents
Protein Kinases
Protein-Serine-Threonine Kinases
Phosphoamino Acids
Cell signaling
Cell adhesion
Immunoprecipitation
Cell Adhesion
Protein Kinase C
Gel Chromatography
Gels

Keywords

  • Detergent resistant complex
  • Ovarian carcinoma
  • Protein kinase activity
  • β1 integrin

Cite this

Association of β1 integrin with protein kinase activity in large detergent resistant complexes. / Skubitz, Amy P; Campbell, Kenneth D.; Goueli, Said; Skubitz, Keith M.

In: FEBS Letters, Vol. 426, No. 3, 24.04.1998, p. 386-391.

Research output: Contribution to journalArticle

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