Association between amino acid sequences in peptide binding region (BOLA-DRB3) and susceptibility or resistance to calf diarrhea

Gh Nikbakht, H. Tadjbakhsh, M. Mokhber-Dezfouli, T. Zahraei Salehi, M. Rabbani, D. Gharibi, M. Emam, F. Assadian, M. Khormali

Research output: Contribution to journalArticlepeer-review

Abstract

The major histocompatibility complex genes (mhc) encode MHC I and II molecules which present peptide fragments to Tcells. Therefore these polymorphic molecules critically influence susceptibility to infectious diseases. At present study potential relationship between amino acid sequences in the antigen binding groove of different BoLA-DRB3 alleles and susceptibility or resistance to calf diarrhea was investigated. Twelve different DRB3 alleles were found among 171 calves (84 diarrheic and 87 healthy) analyesd by PCR-RFLP method. Amino acid sequences of the encoded peptide binding region were compared. 26 polymorphic positions were detected in this region. Asignificant association (p< 0.05) was shown between occurrence of diarrhea and the presence of glutamic acid and tyrosine in pocket 4 and valine, glutamine and leucine in pocket 9 of peptide binding region. Thus it can be concluded that pockets 4 and 9 of the BoLA-DRB3 molecule would be involved in conferring susceptibility of calf to diarrhea.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalJournal of Veterinary Research
Volume66
Issue number1
StatePublished - Aug 26 2011

Keywords

  • BoLA-DRB3
  • Calf diarrhea
  • MHC
  • PCR-RFLP
  • Peptide binding region

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