Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat

S. L. Fox, E. N. Jellen, S. F. Kianian, H. W. Rines, R. L. Phillips

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population. Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes. In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally, the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.

Original languageEnglish (US)
Pages (from-to)320-326
Number of pages7
JournalTheoretical And Applied Genetics
Volume102
Issue number2-3
DOIs
StatePublished - Mar 22 2001

Fingerprint

monosomics
hexaploidy
linkage groups
Restriction Fragment Length Polymorphisms
oats
restriction fragment length polymorphism
Chromosomes
chromosomes
Avena sativa
Avena (Poaceae)
Chromosomes, Human, 6-12 and X
Mothers
Chromosome Banding
Avena
Chromosomes, Human, Pair 16
chromosome banding
DNA
Meristem
cytogenetics
root tips

Keywords

  • Aneuploid
  • Avena
  • Linkage group
  • Monosomic analysis
  • Simulated F

Cite this

Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat. / Fox, S. L.; Jellen, E. N.; Kianian, S. F.; Rines, H. W.; Phillips, R. L.

In: Theoretical And Applied Genetics, Vol. 102, No. 2-3, 22.03.2001, p. 320-326.

Research output: Contribution to journalArticle

Fox, S. L. ; Jellen, E. N. ; Kianian, S. F. ; Rines, H. W. ; Phillips, R. L. / Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat. In: Theoretical And Applied Genetics. 2001 ; Vol. 102, No. 2-3. pp. 320-326.
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