Assessment of transport mechanisms underlying the bicoid morphogen gradient

Brian T. Castle, Stephen A. Howard, David J. Odde

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Morphogen gradients dictate the spatial patterning of multicellular organisms and are established via transport mechanisms. One of the best-characterized morphogens, Bicoid, acts as a polarity determinant in the Drosophila embryo through spatial-temporal control of gap gene expression. The prevailing model for establishment of the gradient has been localized anterior translation, subsequent diffusion, and spatially uniform degradation, consistent with the observed exponential anterior-posterior decay. However, a recent direct measurement of the Bicoid diffusion coefficient via fluorescence recovery after photobleaching (FRAP) resulted in a surprisingly low estimate, which challenged the prevailing model and led to more complicated active transport models. Here, we reassessed this conclusion using a detailed computational model of the FRAP experiment and analysis. In our model, we found disagreement between the input diffusion coefficient and the resulting estimated diffusion coefficient, as measured by previous methods. By using the model to reproduce the original data, we estimate that Bicoid's mitotic diffusion coefficient is 3-fold larger than the originally reported value. Thus, the long-standing diffusive transport model still holds.

Original languageEnglish (US)
Pages (from-to)116-121
Number of pages6
JournalCellular and Molecular Bioengineering
Issue number1
StatePublished - Mar 2011


  • Bicoid
  • Diffusion
  • Drosophila melanogaster
  • FRAP
  • Modeling
  • Monte Carlo simulation
  • Morphogen gradient


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