Assessment of saccharification efficacy in the cellulase system of the brown rot fungus Gloeophyllum trabeum

Jake Tewalt, Jonathan Schilling

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Brown rot fungi uniquely degrade wood by creating modifications thought to aid in the selective removal of polysaccharides by an incomplete cellulase suite. This naturally successful mechanism offers potential for current bioprocessing applications. To test the efficacy of brown rot cellulases, southern yellow pine wood blocks were first degraded by the brown rot fungus Gloeophyllum trabeum for 0, 2, 4, and 6 weeks. Characterization of the pine constituents revealed brown rot decay patterns, with selective polysaccharide removal as lignin compositions increased. G. trabeum liquid and solid state cellulase extracts, as well as a commercial Trichoderma reesei extract (Celluclast 1.5 L), were used to saccharify this pretreated material, using β-glucosidase amendment to remove limitation of cellobiose-to-glucose conversion. Conditions varied according to source and concentration of cellulase extract and to pH (3.0 vs. 4.8). Hydrolysis yields were maximized using solid state G. trabeum extracts at a pH of 4.8. However, the extent of glucose release was low and was not significantly altered when cellulase loading levels were increased threefold. Furthermore, Celluclast 1.5 L continually outperformed G. trabeum cellulase extracts, although extent of glucose release never exceeded 22.0%. Results suggest methodological advances for utilizing crude G. trabeum cellulases and imply that the suboptimal hydrolysis levels obtained with G. trabeum and Celluclast 1.5 L cellulases, even at high loading levels, may be due to brown rot modifications insufficiently distributed throughout the pretreated material.

Original languageEnglish (US)
Pages (from-to)1785-1793
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number6
StatePublished - May 1 2010


  • Bioprocessing
  • Cellulase
  • Ethanol
  • Gloeophyllum trabeum

Fingerprint Dive into the research topics of 'Assessment of saccharification efficacy in the cellulase system of the brown rot fungus Gloeophyllum trabeum'. Together they form a unique fingerprint.

Cite this