Assessing the Effectiveness of Reflectance Spectroscopy Analysis to Determine Ploidy in Potato

Husain Agha, Lucy Schroeder, David Eikholt, Cari A Schmitz Carley, Jeannine Cavendar-Bares, Laura M. Shannon

Research output: Contribution to journalArticlepeer-review

Abstract

While potato is an immensely important crop worldwide, its highly heterozygous, autotetraploid nature limits breeding progress. Converting potato to a diploid, inbred-hybrid crop will allow breeders to respond more quickly to changing environmental pressures and consumer demands. Breeders generate dihaploids by a cross between a cultivated tetraploid potato and a Solanum phureja inducer line, resulting in a reduction in ploidy. This cross has a low frequency of success and results in seeds of unknown ploidy. Here, we present the results of using reflectance spectroscopy analysis as a method to determine ploidy in seedlings following a cross with an inducer line. While our models showed high accuracy in determining ploidy, the specificity was insufficient for spectroscopic analysis to be a viable method for ploidy determination. These data also provide an example which suggests that, while a given phenotype distribution may shift after diploidization, breeding could be effective in making diploids that perform similarly to tetraploid varieties.

Original languageEnglish (US)
JournalAmerican Journal of Potato Research
DOIs
StateAccepted/In press - 2023

Bibliographical note

Funding Information:
The Office for the Vice President of Research at UMN purchased a flow cytometer for us through their Grant in Aid program. This research was funded by USDA-NIFA 2016-34141-25707, USDA-NIFA 2019-34141-30284, USDA-NIFA-SCRI 2019-51181-30021, and the Minnesota Department of Agriculture.

Publisher Copyright:
© 2023, The Potato Association of America.

Keywords

  • Dihaploid
  • Diploidization
  • Machine learning
  • Spectroscopy

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