Assessing the dynamic biofilm removal of sulfonated phenolics using CP-OCT

K. Englund, J. Nikrad, Robert S Jones

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Examining the physical mechanisms related to biofilm removal of sulfonated phenolics (SP) is difficult using conventional microscopy techniques. A custom flow cell system integrated with a real time cross polarization optical coherence tomography system investigated the dynamic speed of biofilm removal when oral multi-species biofilms are exposed to SP under sheer stress. The Near infrared 1310-nm CP-OCT system non-destructively imaged fluid immersed oral biofilms at nearly 30 frames/s. This dynamic imaging was able to determine the cohesive and adhesion related disruption of SP on oral biofilms adhering to tooth like surfaces. For multi-species biofilms that are initially grown without the presence of sucrose, the disruption of biofilms on saliva coated hydroxyapatite (HA) is dominated as a adhesive failure at the HA-biofilm interface. For multi-species biofilms that are grown in the presence of sucrose, the disruption is dominated by cohesive disruption followed by adhesive failure. This novel CP-OCT flow cell assay has the potential to examine rapid interactions between anti-biofilm agents and tooth like surfaces.

Original languageEnglish (US)
Title of host publicationLasers in Dentistry XXIII
EditorsDaniel Fried, Peter Rechmann
PublisherSPIE
ISBN (Electronic)9781510605299
DOIs
StatePublished - Jan 1 2017
EventLasers in Dentistry XXIII - San Francisco, United States
Duration: Jan 29 2017 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10044
ISSN (Print)1605-7422

Conference

ConferenceLasers in Dentistry XXIII
CountryUnited States
CitySan Francisco
Period1/29/17 → …

Keywords

  • Biofilm
  • Biofilm Disruption
  • Dentistry
  • Optical Coherence Tomography
  • Sulfonated Phenolics

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    Englund, K., Nikrad, J., & Jones, R. S. (2017). Assessing the dynamic biofilm removal of sulfonated phenolics using CP-OCT. In D. Fried, & P. Rechmann (Eds.), Lasers in Dentistry XXIII [1004409] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10044). SPIE. https://doi.org/10.1117/12.2254624