Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Vascular endothelial growth factor (VEGF) stimulates vascular permeability in a variety of human pathologies, such as cancer, ischemic stroke, cardiovascular disease, retinal conditions, and COVID-19-associated pulmonary edema, sepsis, acute lung injury, and acute respiratory distress syndrome. Comprehensive investigation of the molecular mechanisms of VEGF-induced vascular permeability has been hindered by the lack of in vivo models that easily facilitate genetic manipulation studies in real time. To address this need, we generated a heat-inducible VEGF transgenic zebrafish model of vascular permeability. Here, we describe how this zebrafish model can be used to monitor VEGF-induced vascular permeability through live in vivo imaging to identify genetic regulators that play key roles in vascular barrier integrity in physiological conditions and human disease processes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages339-350
Number of pages12
Volume2475
DOIs
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by a Grant-in-Aid of Research, Artistry and Scholarship Program Award #380634 from the Office of Vice President of Research at the University of Minnesota, Institutional Research Grant #129819-IRG-16-189-58-IRG81 from the American Cancer Society, and The Hormel Foundation (to L.H.H.).

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Acute lung injury
  • Cancer
  • Cardiovascular disease
  • COVID-19
  • Edema
  • Sepsis
  • Stroke
  • Vascular endothelial growth factor (VEGF)
  • Vascular permeability
  • Zebrafish
  • Capillary Permeability/physiology
  • Zebrafish/genetics
  • Vascular Endothelial Growth Factor A/metabolism
  • Animals
  • Vascular Endothelial Growth Factors/metabolism

PubMed: MeSH publication types

  • Journal Article

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