Abstract
Vascular endothelial growth factor (VEGF) stimulates vascular permeability in a variety of human pathologies, such as cancer, ischemic stroke, cardiovascular disease, retinal conditions, and COVID-19-associated pulmonary edema, sepsis, acute lung injury, and acute respiratory distress syndrome. Comprehensive investigation of the molecular mechanisms of VEGF-induced vascular permeability has been hindered by the lack of in vivo models that easily facilitate genetic manipulation studies in real time. To address this need, we generated a heat-inducible VEGF transgenic zebrafish model of vascular permeability. Here, we describe how this zebrafish model can be used to monitor VEGF-induced vascular permeability through live in vivo imaging to identify genetic regulators that play key roles in vascular barrier integrity in physiological conditions and human disease processes.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 339-350 |
Number of pages | 12 |
Volume | 2475 |
DOIs | |
State | Published - 2022 |
Publication series
Name | Methods in molecular biology (Clifton, N.J.) |
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Publisher | Humana Press |
ISSN (Print) | 1064-3745 |
Bibliographical note
Funding Information:This work was supported by a Grant-in-Aid of Research, Artistry and Scholarship Program Award #380634 from the Office of Vice President of Research at the University of Minnesota, Institutional Research Grant #129819-IRG-16-189-58-IRG81 from the American Cancer Society, and The Hormel Foundation (to L.H.H.).
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords
- Acute lung injury
- Cancer
- Cardiovascular disease
- COVID-19
- Edema
- Sepsis
- Stroke
- Vascular endothelial growth factor (VEGF)
- Vascular permeability
- Zebrafish
- Capillary Permeability/physiology
- Zebrafish/genetics
- Vascular Endothelial Growth Factor A/metabolism
- Animals
- Vascular Endothelial Growth Factors/metabolism
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't