Assembly of MreB filaments on liposome membranes: A synthetic biology approach

Yusuke T. Maeda, Tomoyoshi Nakadai, Jonghyeon Shin, Kunihiro Uryu, Vincent Noireaux, Albert Libchaber

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4-10 μm rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalACS Synthetic Biology
Issue number2
StatePublished - Feb 17 2012


  • Artificial cells
  • Bacterial cytoskeleton MreB
  • Cell-free gene expression
  • Vesicle bioreactor


Dive into the research topics of 'Assembly of MreB filaments on liposome membranes: A synthetic biology approach'. Together they form a unique fingerprint.

Cite this