Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-dependent RNA polymerase, whereas the small (S) genomic segment encodes the nucleoprotein (NP) and the glycoprotein (GPC). The NP encapsidates viral genome, is required for viral transcription and replication, and acts as a type I interferon (IFN) antagonist. This article describes assays to demonstrate that NP contains 3′-5′ exoribonuclease (RNase) activity to degrade modeled RNA of the pathogen-associated molecular pattern type and suppresses the IFNβ promoter-driven luciferase reporter gene. The minigenomic (MG) assay is used to assess the role of NP in replicating and transcribing a viral promoter-driven luciferase reporter gene. These powerful assays demonstrate the versatility of NP in mediating viral replication as well as in modulating host innate immune responses.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages189-200
Number of pages12
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1604
ISSN (Print)1064-3745

Fingerprint

Interferon Type I
Nucleoproteins
Viral RNA
Luciferases
Reporter Genes
Pichinde virus
Lassa virus
Arenavirus
RNA
RNA Replicase
Viral Genome
Ribonucleases
Innate Immunity
Glycoproteins
Genome
Viruses

Keywords

  • Arenavirus
  • Lassa virus
  • Nucleoprotein

PubMed: MeSH publication types

  • Journal Article

Cite this

Huang, Q., Shao, J., Liang, Y., & Ly, H. (2018). Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon. In Methods in Molecular Biology (pp. 189-200). (Methods in Molecular Biology; Vol. 1604). Humana Press Inc.. DOI: 10.1007/978-1-4939-6981-4_13

Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon. / Huang, Qinfeng; Shao, Junjie; Liang, Yuying; Ly, Hinh.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 189-200 (Methods in Molecular Biology; Vol. 1604).

Research output: Chapter in Book/Report/Conference proceedingChapter

Huang, Q, Shao, J, Liang, Y & Ly, H 2018, Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1604, Humana Press Inc., pp. 189-200. DOI: 10.1007/978-1-4939-6981-4_13
Huang Q, Shao J, Liang Y, Ly H. Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon. In Methods in Molecular Biology. Humana Press Inc.2018. p. 189-200. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-6981-4_13
Huang, Qinfeng ; Shao, Junjie ; Liang, Yuying ; Ly, Hinh. / Assays to demonstrate the roles of arenaviral nucleoproteins (NPs) in viral RNA synthesis and in suppressing type I interferon. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 189-200 (Methods in Molecular Biology).
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