Assays to assess arenaviral glycoprotein function

Research output: ResearchChapter

Abstract

Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-dependent RNA polymerase, whereas the small (S) genomic segment encodes the nucleoprotein (NP) and the glycoprotein precursor complex (GPC). GPC is processed by signal peptidase in the endoplasmic reticulum into the stable signal peptide (SSP) and GP1/GP2, which is further cleaved by the Golgi-resident subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P) into the cellular receptor-recognition subunit GP1 and the transmembrane subunit GP2, which helps promote the membrane fusion reaction to allow virus entry into the cell. This article describes assays to assess PICV GPC expression, proteolytic processing, fusion function, and GPC-mediated virus-like particle (VLP) entry into cells under tissue-culture conditions.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages169-178
Number of pages10
Volume1604
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1604
ISSN (Print)1064-3745

Fingerprint

Glycoproteins
Pichinde virus
Lassa virus
Arenavirus
RNA Replicase
Subtilisin
Virus Internalization
Membrane Fusion
Nucleoproteins
Protein Sorting Signals
Endoplasmic Reticulum
Virion
Isoenzymes
Genome
RNA
Viruses
plasma protein Z
site 1 membrane-bound transcription factor peptidase
type I signal peptidase

Keywords

  • Arenavirus
  • Cell entry
  • Cell membrane fusion
  • Glycoprotein precursor complex
  • GPC
  • Lassa virus (LASV)
  • Pichindé virus (PICV)
  • Receptor recognition

MeSH PubMed subject areas

  • Journal Article

Cite this

Shao, J., Liu, X., Liang, Y., & Ly, H. (2018). Assays to assess arenaviral glycoprotein function. In Methods in Molecular Biology (Vol. 1604, pp. 169-178). (Methods in Molecular Biology; Vol. 1604). Humana Press Inc.. DOI: 10.1007/978-1-4939-6981-4_11

Assays to assess arenaviral glycoprotein function. / Shao, Junjie; Liu, Xiaoying; Liang, Yuying; Ly, Hinh.

Methods in Molecular Biology. Vol. 1604 Humana Press Inc., 2018. p. 169-178 (Methods in Molecular Biology; Vol. 1604).

Research output: ResearchChapter

Shao, J, Liu, X, Liang, Y & Ly, H 2018, Assays to assess arenaviral glycoprotein function. in Methods in Molecular Biology. vol. 1604, Methods in Molecular Biology, vol. 1604, Humana Press Inc., pp. 169-178. DOI: 10.1007/978-1-4939-6981-4_11
Shao J, Liu X, Liang Y, Ly H. Assays to assess arenaviral glycoprotein function. In Methods in Molecular Biology. Vol. 1604. Humana Press Inc.2018. p. 169-178. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-6981-4_11
Shao, Junjie ; Liu, Xiaoying ; Liang, Yuying ; Ly, Hinh. / Assays to assess arenaviral glycoprotein function. Methods in Molecular Biology. Vol. 1604 Humana Press Inc., 2018. pp. 169-178 (Methods in Molecular Biology).
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