Assay of netilmicin, using enzyme immunoassay for gentamicin

T. Larson, D. N. Gerding, L. R. Peterson, J. H. Eckfeldt

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Abstract

A homogenous enzyme immunoassay for the quantitative determination of netilmicin in serum was developed. The procedure utilizes a commercially available assay for gentamicin (EMIT; Syva Co., Palo Alto, Calif.). The method was adapted to a microcentrifugal analyzer, and log-logit regression analysis was performed with a computer. The results of samples assayed by this method correlate well with microbioassay (r2=0.985) and radioimmunoassay (r2=0.986). This method is not only precise and accurate, but also very rapid and economical and compares favorably to other available methods of netilmicin assay.

Original languageEnglish (US)
Pages (from-to)399-401
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1982

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