Assay of netilmicin, using enzyme immunoassay for gentamicin

T. Larson, D. N. Gerding, L. R. Peterson, J. H. Eckfeldt

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A homogenous enzyme immunoassay for the quantitative determination of netilmicin in serum was developed. The procedure utilizes a commercially available assay for gentamicin (EMIT; Syva Co., Palo Alto, Calif.). The method was adapted to a microcentrifugal analyzer, and log-logit regression analysis was performed with a computer. The results of samples assayed by this method correlate well with microbioassay (r2=0.985) and radioimmunoassay (r2=0.986). This method is not only precise and accurate, but also very rapid and economical and compares favorably to other available methods of netilmicin assay.

Original languageEnglish (US)
Pages (from-to)399-401
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1982

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Netilmicin
Gentamicins
Immunoenzyme Techniques
Enzyme Multiplied Immunoassay Technique
Radioimmunoassay
Regression Analysis
Serum

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Assay of netilmicin, using enzyme immunoassay for gentamicin. / Larson, T.; Gerding, D. N.; Peterson, L. R.; Eckfeldt, J. H.

In: Antimicrobial Agents and Chemotherapy, Vol. 21, No. 3, 01.01.1982, p. 399-401.

Research output: Contribution to journalArticle

Larson, T. ; Gerding, D. N. ; Peterson, L. R. ; Eckfeldt, J. H. / Assay of netilmicin, using enzyme immunoassay for gentamicin. In: Antimicrobial Agents and Chemotherapy. 1982 ; Vol. 21, No. 3. pp. 399-401.
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