Abstract
A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss of asparaginase II activity. One of the new mutations (and4) was shown to be allelic to gdh-CR, a pleiotropic mutation which causes derepressed synthesis of a number of enzymes of nitrogen catabolism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 293-296 |
| Number of pages | 4 |
| Journal | Journal of bacteriology |
| Volume | 165 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1986 |
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Asparaginase II of Saccharomyces cerevisiae : Selection of four mutations that cause derepressed enzyme synthesis. / Kamerud, J. Q.; Roon, Robert J.
In: Journal of bacteriology, Vol. 165, No. 1, 01.01.1986, p. 293-296.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Asparaginase II of Saccharomyces cerevisiae
T2 - Selection of four mutations that cause derepressed enzyme synthesis
AU - Kamerud, J. Q.
AU - Roon, Robert J
PY - 1986/1/1
Y1 - 1986/1/1
N2 - A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss of asparaginase II activity. One of the new mutations (and4) was shown to be allelic to gdh-CR, a pleiotropic mutation which causes derepressed synthesis of a number of enzymes of nitrogen catabolism.
AB - A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss of asparaginase II activity. One of the new mutations (and4) was shown to be allelic to gdh-CR, a pleiotropic mutation which causes derepressed synthesis of a number of enzymes of nitrogen catabolism.
UR - http://www.scopus.com/inward/record.url?scp=0022481955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022481955&partnerID=8YFLogxK
U2 - 10.1128/jb.165.1.293-296.1986
DO - 10.1128/jb.165.1.293-296.1986
M3 - Article
C2 - 3510190
AN - SCOPUS:0022481955
VL - 165
SP - 293
EP - 296
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -