TY - JOUR
T1 - Asparaginase II of Saccharomyces cerevisiae
T2 - Positive selection of two mutations that prevent enzyme synthesis
AU - Kim, K. W.
AU - Roon, Robert J
PY - 1984
Y1 - 1984
N2 - A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.
AB - A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.
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U2 - 10.1128/jb.157.3.958-961.1984
DO - 10.1128/jb.157.3.958-961.1984
M3 - Article
C2 - 6365897
AN - SCOPUS:0021355041
SN - 0021-9193
VL - 157
SP - 958
EP - 961
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 3
ER -