Asparaginase II of Saccharomyces cerevisiae: Positive selection of two mutations that prevent enzyme synthesis

K. W. Kim, Robert J Roon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.

Original languageEnglish (US)
Pages (from-to)958-961
Number of pages4
JournalJournal of Bacteriology
Volume157
Issue number3
StatePublished - Jan 1 1984

Fingerprint

Asparaginase
Saccharomyces cerevisiae
Mutation
Enzymes
D-Aspartic Acid
Asparagine
Diploidy

Cite this

Asparaginase II of Saccharomyces cerevisiae : Positive selection of two mutations that prevent enzyme synthesis. / Kim, K. W.; Roon, Robert J.

In: Journal of Bacteriology, Vol. 157, No. 3, 01.01.1984, p. 958-961.

Research output: Contribution to journalArticle

@article{5bfc0b636fad4615bf0a4326c9ea1d6a,
title = "Asparaginase II of Saccharomyces cerevisiae: Positive selection of two mutations that prevent enzyme synthesis",
abstract = "A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.",
author = "Kim, {K. W.} and Roon, {Robert J}",
year = "1984",
month = "1",
day = "1",
language = "English (US)",
volume = "157",
pages = "958--961",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Asparaginase II of Saccharomyces cerevisiae

T2 - Positive selection of two mutations that prevent enzyme synthesis

AU - Kim, K. W.

AU - Roon, Robert J

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.

AB - A positive selection method, D-aspartic acid β-hydroxamate resistance, was used to isolate Saccharomyces cerevisiae strains lacking the ability to synthesize asparaginase II. Of 100 such mutant strains, 93 exhibited mutations which were allelic with asp3, a previously characterized mutation. The other seven strains carried a new mutation asp6. The asp6 mutation segregated 2:2 in asp6 x wild-type crosses and assorted from the asp3 mutation in asp6 x asp3 crosses. All seven asp6 mutant isolates reverted at a relatively high frequency, whereas the asp3 mutant isolates did not revert under the same conditions. Various independent asp3 isolates were mated to give heteroallelic diploids, which when sporulated and spread on D-asparagine medium yielded no recombinant strains.

UR - http://www.scopus.com/inward/record.url?scp=0021355041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021355041&partnerID=8YFLogxK

M3 - Article

C2 - 6365897

AN - SCOPUS:0021355041

VL - 157

SP - 958

EP - 961

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -