Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene

K. W. Kim, J. Q. Kamerud, Dennis M Livingston, Robert J Roon

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Purified preparations of asparaginase II of Saccharomyces cerevisiae exhibit two protein bands upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cloning and sequencing of the ASP3 gene, and partial amino acid sequencing as asparaginase II, imply that both bands are encoded by ASP3 but have different N termini. Northern blot analysis using the cloned ASP3 gene as a probe indicates that nitrogen catabolite repression of asparaginase II is achieved by alteration in mRNA levels. Deletion of sequences >600 base pairs upstream from the initiation AUG codon results in an altered response to certain nitrogen sources in strains containing the truncated gene.

Original languageEnglish (US)
Pages (from-to)11948-11953
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number24
StatePublished - Jan 1 1988

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Asparaginase
Yeast
Saccharomyces cerevisiae
Genes
Nitrogen
Catabolite Repression
Initiator Codon
Sequence Deletion
Cloning
Protein Sequence Analysis
Electrophoresis
Base Pairing
Sodium Dodecyl Sulfate
Northern Blotting
Organism Cloning
Polyacrylamide Gel Electrophoresis
Amino Acids
Messenger RNA
Proteins

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Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene. / Kim, K. W.; Kamerud, J. Q.; Livingston, Dennis M; Roon, Robert J.

In: Journal of Biological Chemistry, Vol. 263, No. 24, 01.01.1988, p. 11948-11953.

Research output: Contribution to journalArticle

Kim, KW, Kamerud, JQ, Livingston, DM & Roon, RJ 1988, 'Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene', Journal of Biological Chemistry, vol. 263, no. 24, pp. 11948-11953.
Kim, K. W. ; Kamerud, J. Q. ; Livingston, Dennis M ; Roon, Robert J. / Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 24. pp. 11948-11953.
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