Artificial tritrophic exposure system for environmental risk analysis on aphidophagous predators

Débora Pires Paula, Lucas M. De Souza, David A. Andow, Alex A.T.Cortês De Sousa, Carmen S.S. Pires, Edison R. Sujii

Research output: Contribution to journalArticlepeer-review


We evaluated an artificial tritrophic exposure system for use in ecotoxicological evaluations of environmental stressors on aphidophagous predators. It consists of an acrylic tube with a Parafilm M sachet containing liquid aphid diet, into which can be added environmental stressors. Immature Cycloneda sanguinea, Harmonia axyridis and Chrysoperla externa, and adult H. axyridis were reared on Myzus persicae. Larval and pupal development and survival of all species and reproductive parameters of H. axyridis were similar to published results. The system provides a suitable tritrophic exposure route, enables ex-ante evaluation of stressors, and improves the accuracy of the assessment.

Original languageEnglish (US)
Pages (from-to)1569-1575
Number of pages7
JournalAnais da Academia Brasileira de Ciencias
Issue number3
StatePublished - Sep 2016

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© 2016, Academia Brasileira de Ciencias. All rights reserved.


  • Beneficial insects
  • Biosafety
  • Ecotoxicology
  • Exposure routes


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