Artificial antigen-presenting constructs efficiently stimulate minor histocompatibility antigen-specific cytotoxic T lymphocytes

Liesbeth E.M. Oosten, Els Blokland, Astrid G.S. Van Halteren, Julie Curtsinger, Matthew F. Mescher, J. H.Frederik Falkenburg, Tuna Mutis, Els Goulmy

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Cytotoxic T lymphocytes (CTLs) specific for hematopoietic-restricted minor histocompatibility antigens (mhags) are important reagents for adoptive immunotherapy of relapsed leukemia after allogeneic stem call transplantation. However, expansion of these CTLs to therapeutic numbers is often hampered by the limited supply of antigen-presenting cells (APCs). Therefore, we evaluated whether cell-sized latex beads coated with HLA/mHag complexes HLA-A2/HA-1 or HLA-A2/HA-2 and recombinant CD80 and CD54 molecules can replace professional APCs. The artificial antigen-presenting constructs (aAPCs) effectively stimulated HA-1- and HA-2-specific CTL clones as shown by ligand-specific expansion, cytokine production, and maintenance of cytotoxic activity, without alteration of CTL phenotype. Furthermore, HA-1-specific polyclonal CTL lines were enriched as efficiently by aAPCs as by autologous HA-1 peptide-pulsed dendritic cells. Thus, aAPCs coated with HLA/mHag complexes, CD80, and CD54 may serve as tools for In vitro enrichment of immunotherapautic mHag-specific CTL lines.

Original languageEnglish (US)
Pages (from-to)224-226
Number of pages3
JournalBlood
Volume104
Issue number1
DOIs
StatePublished - Jul 1 2004

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