TY - JOUR
T1 - Artifactual complement activation by blood-drawing apparatus. A clinical and investigational caveat
AU - Hammerschmidt, Dale E
AU - Wilson, N.
PY - 1980
Y1 - 1980
N2 - An increase in the occurrence of false-positive assays for C5a and C3 conversion, combined with hemolytic C assays discrepant between two laboratories, led to the suspicion that serum tubes with silicone separators might activate complement. Plasma incubated with some lots of such tubes showed evidence of complement activation via the alternative pathway, to an extent capable of influencing interpretation of results. The authors recommended that such tubes not be used for C split-product assays, and be used only with caution for routine hemolytic C assays.
AB - An increase in the occurrence of false-positive assays for C5a and C3 conversion, combined with hemolytic C assays discrepant between two laboratories, led to the suspicion that serum tubes with silicone separators might activate complement. Plasma incubated with some lots of such tubes showed evidence of complement activation via the alternative pathway, to an extent capable of influencing interpretation of results. The authors recommended that such tubes not be used for C split-product assays, and be used only with caution for routine hemolytic C assays.
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U2 - 10.1093/ajcp/74.3.308
DO - 10.1093/ajcp/74.3.308
M3 - Article
C2 - 7416082
AN - SCOPUS:0019162774
SN - 0002-9173
VL - 74
SP - 308
EP - 309
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 3
ER -