Articular-cartilage matrix γ-carboxyglutamic acid-containing protein. Characterization and immunolocalization

R. Loeser, C. S. Carlson, H. Tulli, W. G. Jerome, L. Miller, R. Wallin

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42 Scopus citations

Abstract

Matrix γ-carboxyglutamic acid (Gla)-containing protein (MGP) was found to be present in articular cartilage by Westernblot analysis of guanidinium chloride extracts of human and bovine cartilage and was further localized by immunohistochemical studies on human and monkey specimens. In newborn articular cartilage MGP was present diffusely throughout the matrix, whereas in growth-plate cartilage it was seen mainly in late hypertrophic and calcifyingzone chondrocytes. In adult articular cartilage MGP was present primarily in chondrocytes and the pericellular matrix. Immunoelectron microscopy studies revealed an association between MGP and vesicular structures with an appearance consistent with matrix vesicles. MGP may be an important regulator of cartilage calcification because of its localization in cartilage and the known affinity of Gla-containing proteins for Ca2+ and hydroxyapatite.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalBiochemical Journal
Volume282
Issue number1
DOIs
StatePublished - 1992

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