Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli

Kannika Sajjaphan, Nir Shapir, Lawrence P. Wackett, Michael Palmer, Barbara Blackmon, Jeff Tomkins, Michael J. Sadowsky

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Abstract

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.

Original languageEnglish (US)
Pages (from-to)4402-4407
Number of pages6
JournalApplied and environmental microbiology
Volume70
Issue number7
DOIs
StatePublished - Jul 2004

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