Array-based electrical detection of DNA with nanoparticle probes

So Jung Park, T. Andrew Taton, Chad A. Mirkin

Research output: Contribution to journalArticle

1540 Scopus citations

Abstract

A DNA array detection method is reported in which the binding of oligonucieotides functionalized with gold nanoparticies leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticies in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticie probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of ∼ 100,000:1.

Original languageEnglish (US)
Pages (from-to)1503-1506
Number of pages4
JournalScience
Volume295
Issue number5559
DOIs
StatePublished - Feb 22 2002

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    Park, S. J., Taton, T. A., & Mirkin, C. A. (2002). Array-based electrical detection of DNA with nanoparticle probes. Science, 295(5559), 1503-1506. https://doi.org/10.1126/science.1067003